BIOASSAY-GUIDED FRACTIONATION, MECHANISM OF ACTION AND TOXICOLOGICAL EVALUATION OF ANTI-NOCICEPTIVE PRINCIPLES OBTAINED FROM ERYTHROPHLEUM IVORENSE
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2018, Vol 7, Issue 7
Abstract
The present study describes the bioactivity guided identification of anti-nociceptive compounds found in stem bark extract of Erythrophleum ivorense. The stem bark of the plant was air-dried, grounded and macerated using absolute methanol which was then filtered. The filtrate was evaporated using rotary evaporator and Crude Methanol Extract (CME) was obtained. Bioactivity-guided fractionations of CME was performed using partitioning, thin layer, and open column chromatography. Partitioned fractions (20 mg/kg), E. ivorense-fractions (EiF-1 to EiF-6) (20 mg/kg), EiF-1 (5, 10, 20 mg/kg), fractions (F1 to F8) (20 mg/kg), and semi-purified fractions (50 mg/kg) were screened for anti-nociceptive and anti-inflammatory activities. Animals in each group were administered with extract or fractions intraperitoneally. The mechanism of action and toxicological evaluation of semi-purified fraction were also studied. Data were analyzed using One-Way Analysis of Variance (ANOVA) and the level of significance of the data were taken at p<0.05. Partitioned CME yielded butanol (34.7 g), ethyl-acetate (27.0 g), dichloromethane (17.2 g), and n-hexane (6.1 g) extracts. Chromatographic fractionation of ethyl acetate yielded six fractions (EiF-1 to EiF-6). Purified fraction (EiF-1), the only fraction with anti-nociceptive activity yielded sub-fractions (F1 to F8). Screened fractions (F1 to F8) produced F3 as the fraction with highest activities. Further purification of F3 yielded semi-purified fractions coded as F3C1, F3C2, F3C3, F3C4, F3C5-mother liquor (F3C5-MQ) and F3C5-crystal. F3C5-MQ produced a central anti-nociceptive effect while F3C2 indicated peripheral effect. Atropine significantly reversed the anti-nociceptive effect of F3C5-crystal in both phases of formalin test. At the doses studied, the fraction was fairly non-toxic. In conclusion, the semi-purified fractions exhibited peripheral and central anti-nociceptive activities. The central anti-nociceptive effect of F3C5-MQ may be mediated via muscarinic receptor.
Authors and Affiliations
Wakeel O. K.
Keywords
Related Articles
- EP ID EP627434
- DOI -
- Views 127
- Downloads 0
SOLID WASTE MANAGEMENT: A CHALLENGE TO OUR SOCIETYCASE STUDY JHANSI, UTTAR PRADESH
The waste has potential to pose threat to land air and soil. The poor solid waste management leads to social, economic, health and environmental problems. Jhansi is famous for being tourist city of the UP state. The prob...
STUDY ON FRACTURE HEALING ACTIVITY OF ETHNOMEDICINAL PLANTS IN WESTERN NEPAL
Study on biological use of ethnomedicinal plant is of high interest in modern biological research. Researchers are interested in developing new sources of drug that can be from plant origin, which has been used locally b...
LAPAROSCOPIC CHOLECYSTECTOMY UNDER THORACIC SPINAL ANESTHESIA. COMPARATIVE STUDY BETWEEN TWO DIFFERENT HYPERBARIC BUPIVACAINE DOSES
Aims: In our group, after a study showing that spinal anesthesia is safe when compared with general anesthesia, spinal anesthesia has been the technique of choice for this procedure. This is a prospective study with 200...
DRUG UTILIZATION PATTERN IN GERIATRICS: A REVIEW
The age 65 and above is considered as geriatrics. The objective of this study was to assess the drug utilization pattern in geriatrics. The study was primarily targeted at the elderly because, as a group they take more d...
DIOCTAHEDRAL SMECTITE API' ANALYTICAL METHOD DEVELOPMENT AND VALIDATION ON TITRIMETRIC METHOD FOR THE QUANTITATIVE AND QUALITATIVE DETERMINATION OF IN ORAL DOSAGE FORM.
Back Ground[1]: The Dioctahedral Smectite belonging to a Smectite group (derived from the Greek, smectos, which means soap) indicates large chemical inconsistency, consequential in many detailed mineral names (Gwyn 1988)...