Abstract

The fundamental reason for this investigation is to lessen the production cost of cellulase by advancing the generation medium and utilizing an option carbon source such as agro-processing waste deposit. Streptomyces fungicidicus strain RPBS-A4 was subjected to fermentation medium with supplementation of agro-processing waste powder as a solid substrate for cellulase enzyme production. The impact of various process parameters was examined for increasing cellulase production. Cellulase was observed to be produced by S. fungicidicus strain RPBS-A4 using rice bran (5–6%), as best substrate among the tested. Solid state bioprocessing increased the production of enzyme activity at temperature - 40°C, pH - 9.0, inoculum size - 0.6/100 ml, incubation period - 96 h, substrate concentration - 0.6 g/100 ml, carbon source - maltose (1%), and nitrogen source - yeast extract (1%). Under these most favorable conditions, the highest activity 13.18 U/ml of cellulase was observed. The S. fungicidicus RPBS-A4 has been considered as the best cellulase producer in biocatalysis of agro-processing waste deposits.

Authors and Affiliations