Biochemical and Molecular Analysis of Acetylcholine Esterase to Rule out Organophosphorus Detoxification Level in Field Populations of Culex Quiquefasciatus Say
Journal Title: Journal of Communicable Diseases - Year 2019, Vol 51, Issue 2
Abstract
Background & Objectives: Acetylcholinesterase, responsible for neurotransmitter degradation at the cholinergic nerve synapse, is the target of both organophosphate and carbamate insecticides. The current study envisage to assess acetylcholine esterase levels and mutation in the acetylcholine esterase gene1 of Cx. quinquefasciatus of Kozhikode, Cochin, Malappuram, Thrissur, and Palakkad town areas of Kerala, India.Methods: The samples were collected from regularly insecticide spraying area and scarcely spraying area of the five locations in the year 2014, 15 and 16. The assays were done according to the protocol provided in techniques to detect insecticide resistance mechanism; Field and laboratory Manual. Site specific mutation in acetylcholine esterase gene 1 was identified by RFLP using Alu1 enzyme.Results: The % remaining activity in propoxur inhibited fraction in the regularly and scarcely insecticide treating field populations of Cx.quinquefasciatus were higher than 30% and lesser than 30% respectively. The value greater than 30% indicates the chance to develop resistance in field populations. The RFLP using Alu1 on the ace1 also showed the presence of heterozygous genotype in all the five field populations except in Ernakulam where the Cx.quinquefasciatus population possessed homozygous resistant genotype, which is an indication to the excessive and extensive usage of Temephos as a larvicide.Interpretation & Conclusion: The evidence of development of resistance to synthetic insecticides in mosquitoes observed in the present study points to the need of employing new or alternate insecticides which would be easily degradable and have less harmful effect on other organisms
Authors and Affiliations
Anju Viswan K, E Pushpalatha
Keywords
Related Articles
- EP ID EP667747
- DOI 10.24321/0019.5138.201913
- Views 118
- Downloads 0
Effect of Prognostic Factors on Survival Time of Patients of Cardiovascular Disease using Quantile Regression
Background: The study of survival time after the first myocardial infarction among cardiovascular disease patients are quite important for medical practitioners, planners and for the patients. This study tries to study t...
Dengue Vector Surveillance in and around Mormugao Port Trust (MPT) – Goa, India
As per the International Health Regulations, it is a moral responsibility on national governments to keep international seaports/airports and peripheral areas up to 400 meters free of vector mosquitoes in its adult and i...
Indigenous Plants and their Larvicidal Potential against Indian Mosquito Vectors: A Review
All over the world, millions of people are suffering from mosquito borne diseases spreading by bacteria, viruses or parasites and transmitted by mosquitoes to humans. It is estimated that about billions of currencies are...
A Knowledge, Attitude and Practices Survey and Entomological Situation Analysis in Malaria Endemic Tribal Villages of Surajpur District, Chhattisgarh, India
Background and objective: Malaria continues to be a global health concern with 219 million cases in year 2017. Chhattisgarh is a malaria endemic State, which contributes to 13.5% of malaria cases and 18.43% of deaths rep...
Severe Thrombocytopenia and Bleeding Secondary to Acute Viral Hepatitis E
We here report a rare pediatric case of a 12-year-old male, who presented with severe thrombocytopenia and bleeding, and was eventually diagnosed to be having acute hepatitis E. The thrombocytopenia resolved spontaneousl...