Biochemical changes of Culex pipiens females infected with Plasmodium cathemerium
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 6
Abstract
The present work aims to study the biochemical changes of Culex pipiens females infected with Plasmodium cathemerium. For achievement this research, females of Culex pipiens were infected with Plasmodium cathemerium to study the effect of Plasmodium on Culex pipiens females protein profile quantitative and qualitative at 12h, 1d, 4d, 5d and 6d post infection for mid-gut samples, while salivary glands collected after 3d, 4d, 5d, 6d and 7d post infection. The results showed a significant gradual decreases in total protein concentration within the infected samples at all tested times. In case of mid-gut-samples, the total protein concentration of the infected samples decreased on comparing with control samples with exception after 5 d.p.i. the total protein concentration increases, while, in case of salivary glands –samples total protein concentration of the infected samples decreased on comparing with control samples. Protein of Cx. pipiens (control and infected) were separated using Native and SDS-PAGE for mid-gut and salivary glands samples at different times postinfection. The results of native-PAGE demonstrated that there were differences in the overall protein banding pattern in the infected females as compared to control. The results of the SDS-PAGE clarified that the molecular weight of the separated proteins ranged from 494 to 12 KDa for mid-gut samples and from 147 to 8 KDa for salivary glands samples and Plasmodium infection had led to change in the proteins pattern of Culex pipiens mid-guts and salivary glands as compared to control.
Authors and Affiliations
Mohamed Z. Y. Aly, Azza M. M. El gendy, Ebrahem I. I. Mohamed, Salwa I. Sebak
Keywords
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- DOI 10.9790/3008-1306026776.
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