Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
Journal Title: One Health & Risk Management - Year 2021, Vol 2, Issue 3
Abstract
Introduction. It is known that the enzymes catalase (CT) and superoxide dismutase (SOD) are actively involved in protecting cells of microbial pathogens against the biochemical factors produced by macrophages in vivo, in order to stop infection. Therefore, this study was aimed to quantify the parameters of the antioxidant-enzyme activities within the reference cultures under the action of selected new chemical compounds. Material and methods. Cu (II) coordination compounds, Co (II) and Zn (II) were included as “in vitro” study material, as well as aromatic propenones synthesized at the Department of Inorganic Chemistry, at State University of Moldova. The antimicrobial effects were tested on three gram-negative reference strains. The enzymatic activity of SOD and the CT activity was determined to assess the pathogen-induced changes in the antioxidant status under the action of newly tested compounds. Results. The new chemical compounds showed a significant decrease in the antioxidant enzymatic activities of SOD and CT, induced in all reference strains used, which indicates an intense oxidative stress generated by the tested compounds. Conclusions. The specific activity of new chemical compounds on primary antioxidant enzymes, which represent pathogenicity factors of infectious agents, allows us to assume the benefits of in vivo effect of new native substances, as well as to recommend the selected compounds for further biomedical tests.
Authors and Affiliations
Carolina LOZAN-TIRSU, Elena ZARICIUC
Keywords
Related Articles
- EP ID EP696235
- DOI 10.38045/ohrm.2021.3.09
- Views 105
- Downloads 1
Parasitic nematodes in potatoes of different varieties and their interrelations with some arthropods
Introduction. Potato (Solanum tuberosum L.) is susceptible to infestation with an associated complex of different species of parasitic and saprophytic nematodes, bacteria, fungi, and arthropods, which diminish the qualit...
Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60
Introduction. In recent years, due to wide applications of nanotechnologies in various fields, the safety of nanomaterials has become a pressing issue. Fullerene C60 is not an exception. Research on the activity of micro...
Identification of Salmonella spp. strains in poultry products and their impact on Public Health
Introduction. Poultry meat and eggs represent a risk of contamination with zoonotic bacterial agents. This requires permanent laboratory monitoring throughout the production chain segment. Material and methods. The rese...
Major behavioral risk factors for road traffic injuries
Introduction. Road traffic injuries are a major public health problem, ranking 8th in the leading causes of death and are forecasted to rank 5th by 2030 worldwide. Children, pedestrians, cyclists and the elderly remain a...
First national point prevalence survey of healthcare-associated infections and antimicrobial use in acute care hospitals in the Republic of Moldova
Introduction. HAIs present a major public health problem with an impact on morbidity, mortality and quality of life. The objective of the study was to highlight the problem of HAIs and AM use, identification of risk fact...