Biochemical detection of E-ADA on Neospora caninum tachyzoites and the effects of a specific enzymatic inhibitor
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 1
Abstract
Objective. This study aimed to investigate the presence and activity of the ecto adenosine deaminase (E-ADA) enzyme in tachyzoites of Neospora caninum (Nc-1 strain), as well as to assess the activity of a well-known E-ADA inhibitor, the deoxycoformycin. Materials and methods. The parasites were grown in cell culture, being subsequently separated in a pellet of tachyzoites, on which the E-ADA activity was tested using the concentrations 0 (control), 0.2, 0.4 and 0.8 mg mL-1. Results. The E-ADA showed high activity, progressively increasing its activity according to the enhancement of the protein concentration. The test was carried out with different concentrations of deoxycoformycin, showing that it was able to inhibit the E-ADA present on the free form of the parasite. Conclusions. Based on these results we conclude that the E-ADA is present on tachyzoites of N. caninum, and deoxycoformycin is able to inhibit this enzyme. In this sense, knowing the negative impact of N. caninum on reproductive issue in cattle (mainly abortion), might it is an alternative in order to deal with this parasitic infection.
Authors and Affiliations
Alexandre A. Tonin, Ph. D *| Universidade Federal de Santa Maria, Departamento de Clínica de Pequenos Animais, Santa Maria, Brasil.Correspondence: tonin.aat@gmail.com, Aleksandro S. Da Silva, Ph. D| Universidade do Estado de Santa Catarina, Departamento de Zootecnia, Chapecó, Brasil., Victor C. Pimentel, Ph. D| Universidade Federal de Santa Maria, Departamento de Química, Santa Mria, Brasil., Daniele Zanini, Ph. D| Universidade Federal de Santa Maria, Departamento de Química, Santa Mria, Brasil., Maria Rosa C. Schetinger, Ph. D| Universidade Federal de Santa Maria, Departamento de Química, Santa Mria, Brasil., Vera M. Morsch, Ph. D| Universidade Federal de Santa Maria, Departamento de Química, Santa Mria, Brasil., Giovana Camillo, M. Sc| Universidade Federal de Santa Maria, Departamento de Medicina Veterinária Preventiva, Santa Maria, Brasil., Fernanda S.F. Vogel, Ph. D| Universidade Federal de Santa Maria, Departamento de Medicina Veterinária Preventiva, Santa Maria, Brasil., Sonia T.A. Lopes, Ph. D| Universidade Federal de Santa Maria, Departamento de Clínica de Pequenos Animais, Santa Maria, Brasil.
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