Biochemical markers of the functional liver state in rats with toxic hepatitis under the conditions of germanium citrate administration
Journal Title: Біологія тварин - Year 2017, Vol 19, Issue 1
Abstract
Activity of rats’ liver marker enzymes under the conditions of acetaminophen-induced toxic liver injury and influence of germanium citrate was studied. Hepatitis was modeled in these rats by per os acetaminophen administration (1 g/kg in 2 % starch suspension for 2 days using a special catheter). Serum sorbitol dehydrogenase activity was determined by the kinetic method in the reaction of NADH-dependent reduction of D-fructose to D-sorbitol. Serum alanine aminotransferase (ALT) activity and aspartate aminotransferase (AST) was evaluated using a kit of reagents (Filicit-Diagnostica,Ukraine). It is shown that under the conditions of modeled toxic liver injury an increase of the enzymatic activity of sorbitol dehydrogenase, alanine transaminase and aspartate transaminase along with the elevation of De Ritis ratio was observed. Administration of germanium citrate to the intact animals have shown that studied compound is devoid of hepatotoxic properties since the activity of the enzymatic markers of liver state (sorbitol dehydrogenase, AST, ALT) and De Ritis ratio remains on the control level. Study of the hepatoprotector properties of the germanium citrate have shown that in animals with the previously modeled toxic liver injury and administration of germanium citrate a decrease by 2.5times of sorbitol dehydrogenase activity along with the restoration of transaminases activity and De Ritis ratio down to the control values was observed comparing to the animals which weren’t administered with studied compound. Established decrease of the enzymatic activities of marker liver enzymes opens the new perspectives for the study of biochemical mechanisms of the hepatoprotector properties of germanium citrate.
Authors and Affiliations
G. P. Kopylchuk, O. M. Voloshchuk, O. V. Balandyuk
Keywords
Related Articles
- EP ID EP262623
- DOI 10.15407/animbiol19.01.059
- Views 126
- Downloads 0
Crossbreeding as element of high-producing dairy cattle
The review article is devoted to questions of the use of crossbreeding in modern dairy cattle. The analysis of process of breed creation in Ukraine during last decades is made. It is shown that during the last 60 years...
Correction of hygienic activity of bees
The hygienic cleaning of honeybees is a genetic defensive reaction which inhibits the spread of infections on an apiary. The level of its activity determines the protective abilities of bee colonies and can be adjusted b...
Fatty acid composition of tissue lipids goslings and goose embryons
The changes in the fatty acid composition of the lipid in the brain tissues and geese liver and their embryos from 22th embryonic day until 14-th day of postnatal period have been investigated. The fatty acid composition...
Blood biochemical parameters in horses involved in eventing under the influence of exercise
A physical exercise in athletic horses induces series of normal physiological and biochemical adaptations. Eventing is a rigorous exercise challenge, similar to the human triathlon that tests the physical ability and ski...
Surgical management of peroneal nerve paralysis in calf
This article describes a case of peroneal nerve paralysis of left hind limb in 2 month old Holstein calf, weight 60 kg. The final diagnosis of the case was done based on clinical investigation. The severity of the paraly...