Biochemical responses and proline metabolism in Amaranthus tricolor L. and Phaseolus vulgaris L. under in vitro NaCl Stress
Journal Title: Journal of Research in Biology - Year 2011, Vol 1, Issue 4
Abstract
Salinity is currently the major factor which reduces crop yields. One of the biological approaches is to use salt tolerant plants. Amaranthus tricolor L. has been used as a promising plant to ameliorate the salt affected area. The objective of the study is to evaluate the effect of NaCl stress on synthesis and catabolism of proline, soluble proteins, carbohydrates and Na+/K+ ratio in A. tricolor and to compare with the salt-sensitive species, Phaseolus vulgaris L. The experiments were designed with six replications. Seedlings of both species were grown hydroponically with 0, 50, 100, 150, 200, 250 and 300 mM NaCl. The in vitro activity of the enzyme pyrroline 5-carboxylate synthetase under NaCl stress was higher, while, the activity of proline degrading enzyme - proline oxidase showed a reverse trend i.e., low activity in high NaCl concentrations. Soluble protein content was increased in the shoot of A.tricolor but decreased in P.vulgaris. Roots of both the species showed variation in the protein content. Proline content of shoot and roots increased significantly in all the treatments in the plants. However, A.tricolor showed a higher level. The total carbohydrate also showed a similar trend. High level of NaCl decreased the reduced sugar in shoots and roots of the species. Salt stress increased Na+ significantly and decreased the K+ content in both species. The biochemical variation may be interpreted as differential response of the plants to NaCl stress
Authors and Affiliations
Sunukumar SS, Harish SR, Manoj GS, Remya Krishnan, Murugan K
Keywords
Related Articles
- EP ID EP561543
- DOI -
- Views 99
- Downloads 0
Allelopathic effect of aqueous leaf extract of Parthenium hysterophorus on germination and seedling growth of the Gossypium hirsutum
Weeds are sometimes considered as unwanted plants. Some weeds reduce human efficiency through physical discomfort caused by allergies and poisoning. Weeds such as parthenium (Parthenium hysterophorus) and rag weed (Limbr...
Radiosensitizing effects of 2-deoxy-D-glucose and ferulic acid on mouse Ehrlich’s Ascites Carcinoma in Swiss albino mice
The objective of the present study is to evaluate the radiosensitizing activity of combination of 2-deoxy-D-glucose (2DG) and ferulic acid (FA) against Ehrlich’s Ascites Carcinoma (EAC) in Swiss albino mice. Extensive DN...
Effects of dietary energy levels on growth performance, feed utilization and body composition of Rainbow trout
In order to compare the growth performance of trout with two extruded foods and their impact on body composition, an experimental test was conducted from June 1 to October 5, 2010 at National Center of Hydrobiology and F...
Butterfly fauna of Daroji Sloth Bear Sanctuary, Hospet, Bellary District, Karnataka, India
Butterflies were enumerated during February 2010 to January 2012 using pollard walk method to assess the species diversity in the tropical thorn dry deciduous (Deccan Plateau) scrub jungle with granite boulder outcrop ha...
Species diversity and assemblage of fish fauna of Sip River: A tributary of Narmada River
The Sip River is a tributary of the River Narmada, joining Narmada right bank just upstream of Indira Sagar Reservoir. A systematic study of fish diversity in River Sip has been neglected and the information on this aspe...