BIOFILM FORMATION OF METHICILLIN AND VANCOMYCIN RESISTANT STAPHYLOCOCCUS SPECIES ISOLATED FROM CELLULAR PHONES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 3
Abstract
Objective: The goal of the study was to explore the occurrence of methicillin and vancomycin-resistant Staphylococcus species from cellular phones and preventive measures to avoid contamination. Methods: The issues addressed in this investigation are i) Isolation of bacteria from 70 cellular phones through swab culture method ii) Characterization of the isolates by standard biochemical test iii) Antibiogram pattern study of the isolates iv) Molecular identification of the selected isolate through 16s rDNA sequencing v) Virulence study through biofilm assay vi) Susceptibility of the isolates against commonly used disinfectants and essential oils through disc diffusion. Results: All 70 different cellular phone used in this study harbored 120 isolates of bacteria. Of which, we recorded high incidence of Gram-positive cocci (51.6%) and 22 isolates were identified as Staphylococcus species. Three isolates (52N6, 53N9 and 53N6) were resistant to both methicillin and vancomycin, in addition, showed resistance against several other antibiotics. The strain 53N6 that exhibited multidrug resistance was further identified as Staphylococcus sp. strain CPC53N6 through 16s rDNA sequencing with NCBI accession no. KX858346. Virulence activity of the isolate was observed by its biofilm forming capacity as revealed from SEM imaging and the biofilms were composed of aggregated cocci and fibriform extracellular matrix. All the Staphylococcus isolates, including CPC53N6, were sensitive to disinfectants like dettol and cinnamon (Cinnamon zeylanicum) oil indicating their use as preventive measures. Conclusion: Thus, the methicillin and vancomycin-resistant Staphylococcus species of cellular phones can be decontaminated through cinnamon oil to minimize further transmission.
Authors and Affiliations
Swati Pattnaik, Shatabdi Mishra, Chandi Charan Rath, Sudipta Maity, Deviprasad Samantaray
Keywords
Related Articles
- EP ID EP567563
- DOI 10.22159/ijpps.2018v10i3.23051
- Views 91
- Downloads 0
LIGNAN DERIVATIVES POTENTIAL AS PLASMODIUM FALCIPARUM LACTATE DEHYDROGENASE INHIBITORS: MOLECULAR DOCKING APPROACH OF ANTIPLASMODIAL DRUG DESIGN
Objectives: To investigate the lignan derivatives potential as Plasmodium falciparum Lactate Dehydrogenase (PfLDH) inhibitors by using Computer Aided Drug Design (CADD) and molecular docking approach.Methods: In finding...
CYTB: A HOT SPOT FOR PATHOGENIC MUTATIONS IN MITOCHONDRIAL GENOME OF BREAST CANCER AND OVARIAN CANCER PATIENTS
Objective: Out of various cancer types, Breast and ovarian cancers are the most commonly occurring malignancies in women. As per literature, a large number of mutations are reported in various mitochondrial genome encode...
THYMOL TEMPERS As (III) AND Hg (II) CAUSED HYPERCONTRACTILITY BY SIMILAR PATHWAYS IN ISOLATED AORTIC AND TRACHEAL RINGS
Objective: Thymol is known to cause smooth muscle relaxation, possibly by inhibition of Ca2+influx, release or loss of its sensitivity. It is, however, also reported to be a strong antioxidant in several other systems. I...
DEVELOPMENT AND VALIDATION OF LC-MS METHOD FOR QUANTITATIVE ANALYSIS OF A TRADITIONAL THAI ANTIHYPERTENSIVE HERBAL RECIPE
Objective: To develop a new validated LC-MS method in which can be used for identification and quantification of the active pharmaceutical compounds in a traditional Thai antihypertensive herbal recipe (TTAH).Methods: Â ...
FORMULATION, IN-VITRO RELEASE KINETICS AND STABILITY INTERPRETATION OF SUSTAINED RELEASE TABLETS OF METFORMIN HYDROCHLORIDE
Objective: The objective of the present study was to formulate, study the in-vitro release kinetics and stability of sustained release tablets of metformin hydrochloride.Methods: Sustained release formulations that would...