DEVELOPMENT AND VALIDATION OF LC-MS METHOD FOR QUANTITATIVE ANALYSIS OF A TRADITIONAL THAI ANTIHYPERTENSIVE HERBAL RECIPE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: To develop a new validated LC-MS method in which can be used for identification and quantification of the active pharmaceutical compounds in a traditional Thai antihypertensive herbal recipe (TTAH).Methods: Â First, a quadrupole-ion trap MS was used to identify the active pharmaceutical compounds to facilitate a set of markers in the TTAH using multiple reactions monitoring (MRM) method. Coupling to LC in gradients mode, the chromatogram of the TTAH was established. Second, the method validation was conducted to observe several parameters, such as, carryover, linearity, a limit of detection, a limit of quantification, precision, accuracy, robustness, specificity, and system suitability.Results: Piperine, imperatorin, and pinostrobin were identified using the quadrupole-ion trap MS by comparison the mass spectrum of the TTAH samples and reference standards. The parent and product ions were optimized using MRM method. Good chromatographic peaks were achieved along with a simple and fast analysis. All parameters were validated and found that the method reported in this article can be used to quantify the amount of piperine, imperatorin, and pinostrobin in the TTAH.Conclusion: Piperine, imperatorin, and pinostrobin were selected as the markers in LC-MS analysis of the TTAH. This new validated LC-MS method is readily to use in a quality assurance of the TTAH.
Authors and Affiliations
Tossaton Charoonratana, Kingkan Bunluepuech, Tun Chusut, Jirapornchai Suksaeree, Thanapat Songsak
Keywords
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