COMBINED EFFECTS OF CADMIUM AND MECURY ON SOME BIOCHEMICAL AND HISTOCHEMICAL CHANGES IN LIVER, KIDNEY AND GILLS OF CHANNA PUNCTATUS (BLOCH)
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 8
Abstract
Objective: The present study was conducted to investigate the effects of cadmium (Cd) and mercury (Hg) in combination at sub-lethal concentrations for 32 days on histochemical localization of heavy metals and on serum biochemical parameters such as glucose, triglyceride, cholesterol and total protein concentrations in Channa punctatus.Methods: Biochemical estimations of serum glutamic-pyruvic transaminase (SGPT), glucose and different lipids were done using the standard protocols provided in the commercial kits purchased from Reckon diagnostics Pvt. Ltd., India. Histochemical analyses of liver, kidney and gills were determined by sulphide–silver method and heavy metal (cadmium and mercury) concentrations in gills, kidneys and liver were analyzed by Atomic Absorption Spectroscopy.Results: Glucose, lipid, total protein and SGPT levels were significantly altered in fish exposed to Cd or Hg salt alone. However, combined exposure of Cd and Hg normalized all the aforesaid biochemical indices. The accumulation of heavy metals was in following order: cadmium content was more in kidney>liver>gills; whereas, Hg content was more in liver>kidney>gills. Histochemical analyses revealed that mercury accumulation was comparatively more than that of cadmium in all the tissues. Several histological abnormalities were noted in the liver, kidney and gills of the Cd or Hg treated animals. However, Cd in combination with Hg caused alleviation in the toxic effect of Hg on histochemical and biochemical parameters. Results of our study showed that Cd may have a protective effect against Hg toxicity.Conclusion: Our findings indicate that cadmium may protect mercury-induced toxicity.Â
Authors and Affiliations
Alpana Arya, Guru Dutta Sharma
Keywords
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