Biofilm resistance to antimicrobial agents by Streptomyces sp.
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2015, Vol 1, Issue 1
Abstract
The study focused about mainly the isolation of Streptomyces species, a group of Actinomycetes from soil samples collected from various places of both Tamil nadu and Kerala. Soil samples were initially serially diluted and plated on Actinomycetes isolation agar and incubated at 280c for 5-7 days and then in ISP 5 medium incubated at 28O c- for 7- 14days. About 30 Actinomycetes isolates were initially isolated and among them only five actinomycetes were screened by cross streak method against specific stranded strains. They are characterized and grouped to the genus of Streptomyces by their colony morphology, spore morphology, growth on selective media and the biochemical characteristics. The presence of polyunsaturated fatty acids were examined in all potential strains by PUFA analysis. The potential strains were mass cultivated in starch casein broth at 24oC for 4- 7 days. S7 strain produced orange colored diffusible pigment in the starch casein broth The biofilm study was conducted by crystal violet assay by using stranded stains. The microtiter plate method was done in 12- well polysterene plate and the crystal violet assay was subjected to determine the density of cells by reading at UV spectrophotometer at 490nm. Dense biofilm was observed in Staphylococcus sp. and pseudomonas sp. And medium in other bacterial strains . Minimum inhibitory concentration testing (MIC 50) of five different actinomycetes supernatant were determined. MIC50 of S7 was found to be as 150 µl for Staphylococcus sp., 200 µl for Pseudomonas sp., for E.coli and Bacillus it was observed as 100 and 75 µg/ml concentrations. Biofilm inhibitory concentration testing: at 300 µl concentration inhibition was observed as which means that the S7 concentration for biofilm was greater than sessile or planktonic cells. Silver nanoparticles was produced from the highly potential strain S7 and its antibiogram was noted .
Authors and Affiliations
P. Ragul H. Sorna Kumari
Keywords
Related Articles
- EP ID EP575536
- DOI -
- Views 117
- Downloads 0
An Additional Tool for Precise Location of the Chiasma Plantare: A Morphometric Study on Fresh Frozen Cadavers
In this study, the distances between the chiasma plantare and two bony landmarks (summit of the medial malleolus and tuberosity of the navicular bone) were measured. In addition, the angle between these structures was me...
Prevalence of Hepatitis C in alcoholic liver disease.
Alcohol consumption and hepatitis C virus (HCV) infection have a synergic hepatotoxic effect, and the coexistence of these factors increases the risk of advanced liver disease. The main mechanisms of this effect are incr...
Effect of Gender on Cardiovascular Response at 12.5% of Maximum Voluntary Contractions of Dynamometer
Introduction: Handgrip device held at 30% of maximum voluntary contractions (MVC) is one among the batteries of test to assess the sympathetic autonomic nervous system (ANS). Subjects at our set-up failed to continue con...
Schiff bases: An emerging potent class of pharmaceuticals
Schiff bases are the utmost extensively used organic compounds. An outsized number of Schiff bases and their complexes have been investigated for their fascinating and imperative properties. Schiff bases are significant...
A cross sectional study on usage of Ennvagaithervu for diagnosis among post graduate scholars at Government Siddha Medical College Hospital, Palayamkottai
Background: The Siddha system of medicine is an ancient system of medicine and it has unique diagnosis method “Ennvagaithervu” as Naa, niram, mozhi, vizhi, sparisam, malam, moothiram and naadi. Insufficient studies noted...