BIOINFORMATIC CHARACTERIZATION OF SOD UNDER UV IN FENUGREEK (FAMILY: FABACEACE)
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 3
Abstract
Objective: To perform In silico analysis of superoxide dismutase (SOD) of the Fabaceae family along with the assessment of its physiological significance under UV irradiation in fenugreek belongs to the same family.Methods: In silico study was carried out for the superoxide dismutase of Fabaceae and isozyme expression in the fenugreek was measured subjected to UV exposure during the post-germination phase.Results: The expression of the enzyme was found constitutive in both the treated and control sets. On gel analysis exhibited a significant enhancement in the expression of enzymes under UV exposure especially at 365 nm. Computational analysis of SOD of Fabaceae revealed that the physicochemical properties and secondary structural features of the isoforms differ to some extent. It was also found that the motif sharing pattern and the conserved domain were different for each isoform, which may be attributed to the difference in cofactor involved.Conclusion: The results suggest that the elevation of SOD activity might be responsible towards overcoming the stress imposed by UV irradiation. Thus, the role of SOD may be beneficial for oxidative stress management in plants during germination phases.Keywords: In-silico Analysis, Superoxide dismutase, Trigonella foenum-graecum, UV irradiation
Authors and Affiliations
Saran Kumar Gupta, Palash Mandal
Keywords
Related Articles
- EP ID EP577797
- DOI -
- Views 49
- Downloads 0
EMULSIFYING PROPERTIES OF AFZELIA GUM IN LIQUID PARAFFIN EMULSION
Objective: Afzelia africana gum has been shown to possess surface activity. It is a good alternative to sodium carboxy methylcellulose in terms of suspending properties. This work was aimed at evaluating the emulsifying...
FAST CHIRAL HPLC PROCEDURE FOR THE SIMULTANEOUS DETERMINATION OF DROPROPIZINE ENANTIOMERS AND ITS NONPOLAR IMPURITY IN RAW MATERIAL AND PHARMACEUTICAL FORMULATION
Objective: Levodropropizine is a novel antitussive drug, which occurs as enantiomers. They are levodropropizine (2S) [LDP] and dextrodropropizine (impurity A) (2R) [DDP]. An isocratic chiral high performance liquid chrom...
DEVELOPMENT OF HPLC AND CHEMOMETRIC ASSISTED SPECTROPHOTOMETRIC METHODS FOR THE SIMULTANEOUS DETERMINATION OF FIVE ACTIVE INGREDIENTS IN COUGH AND COLD TABLETS AND THEIR APPLICATION TO DISSOLUTION STUDY
Objective: Chemometric assisted spectrophotometry, and HPLC methods have been developed for the simultaneous determination of phenylephrine hydrochloride (PEPH), paracetamol (PCM), guaifenesin (GNF), chlorpheniramine mal...
PHARMACOKINETIC DRUG INTERACTIONS OF GLICLAZIDE AND ITOPRIDE IN NORMAL AND DIABETIC RATS
Objective: The present study was aimed to investigate the safety, reliability of Gliclazide and possible drug interaction with Itopride when they were administered as combination treatment.Methods: Studies were conducted...
IN VITRO EVALUATION OF THE NON-CYTOTOXIC THYMUS CAPITATUS EXTRACTS INHIBIT BOVINE HERPESVIRUS-1 EXPANSION
Objective: Bovine herpes virus type 1 (BHV-1) is an important cofactor in the bovine respiratory disease complex with high health and financial impact because there aren’t any available drugs that proved to be fully ef...