BIOLOGICAL ACTIVITY OF EXTRACTS FROM THE MYCELIUM OF MEDICINAL MUSHROOM INONOTUS RHEADES
Journal Title: International journal of ecosystems and ecology science (IJEES) - Year 2018, Vol 8, Issue 3
Abstract
The search for bioactive natural compounds potentially having antitumor activity - important problem of modern science. We have previously demonstrated high in vitro antiradical activity of water ethanol extracts (WEE) from the mycelium of Inonotus rheades. In the present study we evaluated the effect of 30% and 70% of WEE from mycelium I. rheades on the cell culture of human tumor cells HEp-2. 50% cell death is achieved after 24-h incubation in 53 µg/ml 70% WEE (dry weight), whereas 30% WEE at 550 µg/ml only, i.e. an order of magnitude difference between the concentrations, which indicates that high cytotoxic activity was shown WEE a radical change in the qualitative composition of extractives with increasing ethanol concentration. Incubation of cells with 250 mM H2O2 resulted in approximately 40% cell death, whereas pre-incubation with both 30% and 70% of WEE resulted in 100% survival of cells in a large range of concentrations. This indicates a WEE protective effect under oxidative stress. Thus, WEE of the mycelium I. rheades in high concentrations exhibit cytotoxic activity. At the same time, a wide range of concentrations extracts can neutralize the oxidative stress and cell death caused by the addition of H2O2.
Authors and Affiliations
Borovskii G. B. 1,2*, Borovskaya M. K. 1,2, Gornostay T. G. 1
Keywords
Related Articles
- EP ID EP282240
- DOI 10.31407/ijees837
- Views 105
- Downloads 0
DETERMINATION OF PHENOLOGICAL PERIODS OF TREES BY THE METHOD OF CONDUCTOMETRY
The electrical conductivity of the near-cambium layer of four tree species in the Southern Baikal region was measured for two years: Pinus sibirica Du Tour., Abies sibirica Ledeb., Betula pendula and Picea obovata var. c...
THE INTEGRATING ECOLOGICAL URBAN ENVIRONMENTAL PERFORMANCE CRITERIA IN URBAN DESIGN STUDIOUS OF UNIVERSITY STUDENTS
The uncontrolled urbanization caused significant changes in urban environmental conditions due to increased build-up areas of cities, reduction the urban landscape, disruption of human-environment relations. The urban en...
AN EXPERIMENTAL INVESTIGATION OF BIOGAS PURIFICATION WITH GRANULATED SLAG UNDER DIFFERENT PRESSURES
Biogas, with its production and energy potential, is an important renewable energy source for the developing renewable energy market. Biogas is a type of fuel that is produced from the biochemical degradation of biomass....
COMBINATION ABILITY STUDY OF SEVERAL LINES SYNTHESIZED BY AGROARFA ALBANIA BASED ON THE TEST METHOD
Creating new lines and hybrids, with higher production capabilities than existing ones, suitable for particular ecological and agricultural environments, remains a continuing problem ahead of the maize breeding programs....
EVALUATION OF GI BLEEDING PREVALENCE AND ITS RELATED FACTORS IN DIABETIC PATIENTS HOSPITALIZED IN KHATAM-OL-ANBIA HOSPITAL DURING 2015-16: A RETROSPECTIVE STUDY
Introduction: Diabetes is the most common disease due to metabolic disorders and has been considered as a global challenge. It is a chronic, metabolic and genetically heterogeneous disease characterized by elevated of bl...