Biosynthesis of silver nanoparticles using Bacillus sp. for Microbial Disease Control: An in-vitro and in-silico approach
Journal Title: Scholars Academic Journal of Pharmacy - Year 2015, Vol 4, Issue 9
Abstract
The development of reliable and eco-friendly organisms of silver nanomaterials is an important aspect of current nanotechnology research and application. In current study, we report extracellular biosynthesis of metallic silver nanoparticles at two molar concentrations (1mM and 5mM) of AgNO3 using Bacillus sp. isolated from solid waste soil sample. The potential culture was isolated on nutrient agar medium and identified by several staining and biochemical tests. The potential isolate capable of reducing AgNO3 was identified as Bacillus sp. The reduction of AgNO3 was monitored by visible colour change of the solution. UV-Vis spectrum of silver nanoparticles (1mM and 5mM) obtained from the above bacteria containing silver ions showed a peak around 393nm and 405nm respectively. These nanoparticles (1mM and 5mM) were processed for antimicrobial activity and analyzed for plastic degradation. 5mM concentration of silver nanoparticles showed better results against Pseudomonas sp., Staphylococcus sp., and Aspergillus niger compared to 1mM concentration. Silver nanoparticles showed good antibacterial effect against Klebsiella sp. at 1mM concentration. Whereas, Candida albicans was found to be resistant to silver nanoparticles. Efficacy of the silver nanoparticles in degradation of plastics was analyzed by liquid culture method. Silver nanoparticles and bacteria degrade 18% of plastics and silver nitrate 0% of plastics after 15 days period; this reveals that formed silver nanoparticles were not having enhancing role in the plastic biodegradation as compared to microorganism. In-silico analysis was performed to reveal the protein-protein network and functional partners of silver nitrate reductase of Bacillus sp.
Authors and Affiliations
Shivani Tiwari, Jyotsna Gade, Anjali Chourasia,, Aruna J, Geetha GR, Kumar KM
Keywords
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