Bone Marrow Iron Stores among Various Hematological Disorders
Journal Title: Indian Journal of Pathology: Research and Practice - Year 2017, Vol 6, Issue 1
Abstract
Background: Nutritional anemia, particularly iron deficiency, continues to be a major public health problem worldwide, particularly in the developing countries like India. Although mass spectrometry has been recently used to give a definitive determination of iron in tissue, microscopic examination of Prussian bluestained bone marrow aspirate has been considered the practical “gold standard” for determining iron depleted states. Aim: To assess the bone marrow iron stores among the various haematological disorders. Materials and Methods: A cross sectional observational study was conducted by the department of pathology at Shri B.M. Patil Medical College, Hospital and Research centre, Vijayapur for a period of one year. A total of 110 cases of various hematological disorders were studied to assess the bone marrow iron stores. Bone marrow aspirate was obtained after written informed consent from the posterior superior iliac spine observing strict asepsis, spread onto slides, air dried, fixed with methanol, and stained with Giemsa Stain, observed microscopically and also simultaneously one smear stained with Prussian blue stain. Equal volumes of 2% of potassium ferrocynide and 2% hydrochloric acid solution are mixed in staining jar and slides are immersed in the solution for 1520 min. Then removed and rinsed with tap water. Counterstaining was done with 1% neutral red for 30 seconds. Results: Of the various haematological disorders 61.7% of the RBC disorders and 60% of the combined disorders had shown decreased bone marrow iron store, whereas among WBC disorders and platelet disorders it was 45% and 33.3% respectively and there was no statistically significant association (p=0.461) between any of the haematological disorder and the reduced bone marrow iron stores. Conclusion: Performing a bone marrow study in all patients with haematological disorders should be made routine before starting iron replacement therapy.
Authors and Affiliations
Shruti V. Kulkarni
Keywords
Related Articles
- EP ID EP462146
- DOI 10.21088/ijprp.2278.148X.6117.13
- Views 97
- Downloads 0
Expression of Cathepsin D in Pancreatic Carcinoma
Background: Carcinoma of the Pancreas is one of the leading causes of mortality due to malignancy. Most of the cases present with advanced stage because of subtle clinical manifestation. With a curative resection, the fi...
Significance of Nuclear Morphometry in Evaluation of Squamous Intraepithelial Lesions in Vaginal Cytology: A Case Series Study
Context: Cervical cancer is the second leading malignancy in the women occurring worldwide. Researches based on nuclear morphometry on cervicovaginal smears are very much sparse; hence we have taken up this study. Aims:...
Evaluation of Fluorescent Staining for the Diagnosis of Leprosy in Tissue Sections and its Histopathological Correlation : Comparision with ZN Staining
Introduction: to study histopathological evaluation and its correlation with fluorescent staining used in the diagnosis of leprosy in tissue section and comparision of fluorescent staining with zn staining. Materials and...
Holotranscobalamin : A Newer Parameter in Diagnosis of Vitamin B12 Deficiency States
Background and objectives: Vitamin B12 is an essential nutrient that plays crucial roles in the human body. B12 deficiency primarily affects the hematopoietic system, but its effects extend to other tissues and organs, m...
Antenatal Screening for Haemoglobinopathies by Using High Performance Liquid Chromatography
Clinical manifestation of Haemoglobinopathies may vary from asymptomatic state to severe, lifelong, transfusion dependant anemia and reduced life expectancy. About 1.1% of couples around the world are at risk for having...