Brewer’s yeast resistance to Xray radiation
Journal Title: MedPulse -International Medical Journal - Year 2016, Vol 3, Issue 8
Abstract
Be it the repair of genetic damage to humans using Enzymatic Photolyase or the adverse consequences of radiation therapy, Brewer’s resistance to x-ray radiation is crucial to comprehending and developing these concepts. The experiment sheds light on the fact that, though genetic damage may occur in the short run, periods of rest allow the continuation of normal metabolic activity as genetic damage is reversed over time. Brewer’s Yeast is formed from a single celled fungus known as Saccharomyces cerevisiae. Today, Brewer’s Yeast remains to be one of the most commonly used experimentation subjects due to their rapid growth periods and easy accessibility. Their genetic structure has made them one of the most crucial components in modern evolutionary research. Grown in an insulated dish with adequate water and sucrose, the yeast rapidly develops into large defined colonies, making them a capable indicator of structural changes as a result of external stimuli. Their reproductive process is known as budding in which the parent organism develops an external growth that further branches off into a new organism. Yeast has been critical to the development of human biology as well. It is through these cells that we have developed a functional understanding of the cells which control the cell cycle, cell apoptosis, signaling proteins, and protein processing enzyme. Two Control Groups - A and B - were not exposed to any radiation and they contained yeast contained in a petri dish for the duration of the experiment. Experimental group - C1, C2, C3 and C4 - contained yeast that was exposed to 20 kilovolts of X-ray radiation for 10 seconds and 5 times over 5 Days. Experimental group - D1, D2, D3 and D4 - contained active yeast that was also exposed to 20 kilovolts of radiation for 10 seconds, 5 times over 10 days. Experimental Group - E1, E2, E3 and E4 - will be exposed to 20 kilovolts of radiation for 10 seconds every third day for 15 days. The holistic effect on Brewer yeast was then analyzed.
Authors and Affiliations
Prerit Kothari, Nidhi Patni
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