Brine shrimp lethality assay test, Insilico-molecular Docking studies as a preliminary screening models of some newly synthesized 2-substituted 4,6 dichloro 1,3,5, triazine chalcone hybrids for potential cytotoxic activity
Journal Title: Saudi Journal of Medical and Pharmaceutical Sciences - Year 2017, Vol 3, Issue 3
Abstract
Abstract: An attempt was made in the present study to test for in vivo Brine Shrimp Lethality Assay (BSLT) and molecular docking studies of some newly synthesized 2-substituted 4,6 di chloro 1,3 triazine-chalcone hybrids as potential cytotoxic gents. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). Thirty nauplii were added into three replicates of each concentration of the synthesized compounds. After 24 hours the surviving brine shrimp larvae were counted and LC50 was assessed. Schrodinger Mastero docker was used for insilico molecular docking studies on selected cancer protein Targets. The results drawn from the preliminary studies showed that the compounds TCH-9, 12 and 20, 24 were potent against the brine shrimp with minimum LC50 values of 3.125, and 6.25μg/mL respectively. These findings suggest that the synthesized 1,3,5 triazine chalcone hybrids possess potential cytotoxic activity. Keywords: Brine shrimp lethality assay, LC50, molecular docking, Triazine chalcone hybrids
Authors and Affiliations
Venkata Pavan kumar G, Pooja. Boyapati
Keywords
Related Articles
- EP ID EP390325
- DOI -
- Views 94
- Downloads 0
A Study on Acid – Base Indicator Property of Flowers of Leucas zeylanica
Indicators are used to determine the equivalence point in acid – base titrations (neutralization titrations). They show sharp color change with respect to change in pH. Popularly used indicators for neutralization titrat...
Knowledge and Practice of Nurses about Needle Stick Injury at Lahore General Hospita
Abstract: Needle stick injuries are wounds caused by needles that accidently harm the skin. These wounds are harmful for individuals who work with needles and other sharp equipments. These wounds can happen whenever indi...
Impact of Counseling in End Stage Renal Disease Patients of Riyadh (KSA)
Abstract: Lack of patient’s awareness on the importance of adherence to drug regimen is implicated as one among the several factors affecting patient safety. The impact of non-compliance increases substantially in compro...
Evaluation of Chronic Haematological and Neurological Effects of Organophosphates Pesticides Exposure in Children of Rural Population
Abstract:This is an award-winning study of its kind first time conducted in the rural areas of Indore Madhya Pradesh with the aim of evaluating the subclinical morbidity and mortality pattern of the chronic hematological...
Knowledge, Attitudes and Practices on Medication Use and Safety among Saudi People: a Public –based Versus an Internet –based Survey in Taif; Kingdom of Saudi Arabia
Abstract: Suboptimal knowledge on medication use and safety and negative attitudes has negative impact on drug use. The major objective of this study was to measure the public knowledge and to identify their attitudes an...