Buccal Cell Micronuclei Assay: A Non-Invasive Genotoxic Marker
Journal Title: International Journal of Contemporary Medical Research - Year 2017, Vol 4, Issue 1
Abstract
The introduction of non-invasive and presumptive diagnostic techniques for oral premalignant and malignant lesions in an early stage gave rise to the need for Buccal Cell Micronuclei assay. Formation of micronuclei due to carcinogenic agents depicting cytogenic damage forms the basis of this assay. During nuclear division in cell replication cycle, the chromosomal fragments which fall short from participation in new daughter cell nuclei formation, give rise to micronuclei. These may be clastogenic and aneugenic depending on presence or absence of centromere. Similarly, other nuclear abnormalities like pyknotic cells, binucleated cells, karyorrhectic cells, condensed chromatin may indicate towards pending genomic instability leading to major or minor degenerative or developmental diseases. The knowledge regarding cytotoxicity of several genotoxins and their affliction with micronuclei formation has been discussed in detail. This review summarise the methodology and result analysis of the test including the various stains that can be utilised for buccal cell micronuclei assay. It also highlights various studies done on BCMN assay in various lesions and subjects exposed to various genotoxic agents and also the futuristic aspects in this assay.
Authors and Affiliations
Vipul Jain, Parul Lohra, Bhanu Priya, Deepak Sindhu
Keywords
Related Articles
- EP ID EP425303
- DOI -
- Views 110
- Downloads 0
Synovial Sarcoma- A Review
Synovial sarcoma is an uncommon soft tissue neoplasm, whose origin is very controversial. Clinically, it shows a very aggressive behavior, histopathologically, it presents as a biphasic tumor displaying epithelial and sp...
Treatment Outcome of Second Line Anti-retroviral Therapy in HIV Positive Patients Over One Year Period
Introduction: In India, the second line regimen has still not been studied extensively when compared to it’s first line counterpart. In our study we accessed the clinical, virological and immunological effectiveness and...
Assessment of Intraocular Pressure and Visual Acuity in Patients of Lens Induced Glaucoma before and after Manual Cataract Surgery
Introduction: Lens induced glaucoma is commonly come across in elderly people if not treated early and promptly leads to poor visual prognosis and adds to old age disabilities. The aim of this study was to clinically ass...
Comparative Study of Bacterial Profile of Patients with Bacterial Corneal Ulcer with Bacterial Profile of Conjunctiva of Controls - A Prospective Study
Introduction: The normal ocular flora has an essential role in preventing as well as causing ocular infection. In addition to the other ocular parts, conjunctiva harbour natural flora which might play an important role i...
A Study of Serum Lipid Profile among Obese and Non-Obese Individuals: A Hospital based Study from Karbala, Iraq
Introduction: Obesity is a multifactor disorder and its development is due to multiple interactions between genes and environment. This study aims at measuring and correlating values of serum triglyceride level in obese...