Buffalo genome research - a review
Journal Title: Animal Science Papers and Reports - Year 2014, Vol 32, Issue 3
Abstract
Buffalo (Bubalus bubalis and Bubalus carabanesis) are important animals in livestock and agricultural economy of many countries, providing milk, meat and hides along with draught power. But, despite of immense advantages buffalo is a heavily neglected animal. The real potential of buffalo has never been realized and challenges such as reduced reproductive efficiency still needed to be addressed. At the onset of genomic era, after sequencing of entire cattle genome and recently sequencing of buffalo genome, there is a start of new chapter of buffalo research. This review is focused to highlight the recent advances in genome science of buffalo with respect to cytogenetic studies, whole genome mapping and application of next generation sequencing. These resources of genome science can be applied to provide knowledge and technologies to enhance the production potential to the optimum level, improve reproduction efficiency and increase disease resistance in buffalo. Buffalo and domestic cattle belong to same family and are closely related on phylogenetic tree. Therefore, available cattle genetic and genomic resources can be used as shortcuts by researchers to explore genome of buffalo and apply biotechnology tools for buffalo improvement.
Authors and Affiliations
Muhammad Moaeen-ud-Din
Population of ciliates, rumen fermentation indicators and biochemical parameters of blood serum in heifers fed diets supplemented with yeast (Saccharomyces cerevisiae) preparation
Evaluated was the influence of live cells and metabolites of yeast (Saccharomyces cerevisiae) in the diet on number of ciliates, concentration of volatile fatty acids (VFA) in the rumen and biochemical parameters of bloo...
A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White pigs
The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolat...
Effect of exogenous glutathione in extender on the freezability of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa
The study was designed to investigate the effect of exogenous glutathione supplementation (0.0, 0.5, 1.0, 2.0 and 3.0 mM) in tris-citric acid extender on post-thaw sperm motility, viability, plasma membrane and acrosomal...
Prokaryotic expression, purification of chicken calpastatin protein and production of calpastatin polyclonal antibody
The open reading frame of chicken calpastatin (CAST) gene composed of 2,301 base pairs was ligated into a prokaryotic expression vector pET21a (+) to yield pET21a - CAST. The C-terminal His-tagged CAST protein was then e...
HMGR messenger RNA and protein expression in liver, kidney and muscle in pigs of two breeds
Distribution and expression of the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) in different breeds and tissues of pigs were studied by means of immunohistochemical and RT-PCR methods. Three Huai and three Landrace pi...