Immunohistochemical distribution of c-Kit and its gene expression via real-time PCR in skin tissue of goats with a black and white coat colour
Journal Title: Animal Science Papers and Reports - Year 2016, Vol 34, Issue 3
Abstract
The signaling pathway of the stem cell factor and its receptor, c-Kit, plays an important physiological role in melanogenesis. The expression of c-Kit was investigated using real-time quantitative PCR and immunohistochemical staining in skin with a white and black coat colour. The length of the entire open reading frame of the c-Kit gene is 2925 bp. The c-Kit protein is conserved in the structure domain. The c-Kit is mainly located on membranes and widely expressed in the skin tissue. The mRNA expression of c-Kit is 1.28 times higher in skin with the black coat colour than in that with the white coat colour. There was no significantly different expression of c-Kit mRNA and protein (P>0.05) between skin with the white and the black coat colour. These results indicate that there seems to be no direct relationship between coat colour and the expression of the c-Kit gene in goats.
Authors and Affiliations
Zhou Rongyan, Wei Yanhui, Xi Jianzhong, Zhang Junjie, Ma Mengyun, Chen Hui, Li Xianglong
Keywords
Related Articles
- EP ID EP177778
- DOI -
- Views 131
- Downloads 0
Polymorphism and structure of exon 2 of caprine melatonin receptor 1b gene and its relations to fertility and seasonal estrus
The first description is presented of nucleotide sequence of exon 2 of caprine melatonin receptor 1b (MT2). No polymorphisms of MT2 gene were detected between high fertility and year-round estrous goat breeds and low fer...
Changes in proteins and tenderness of meat from young bulls of four breeds at three ages over 10 days of cold storage
The study aimed at analysing the changes taking place in the muscle tissue of cattle during meat cold storage in relation to animal genotype (breed) and age. Investigations were conducted on the thoracic and lumbar par...
Cytogenetic, histological, hormonal and semen studies in male goats with developed udders
Cytogenetic, hormonal, histological and semen quality examinations were performed in two unrelated male goats with developed udders (gynaecomastia). The bucks were neither stimulated for lactation nor milked. The progres...
The polymorphism of bovine FIT gene and its associations with cattle (Bos taurus) growth traits
Fat-inducing transcript (FIT) are endoplasmic reticulum-resident membrane proteins that induce lipid droplet accumulation. It plays a crucial role in the fundamental process of storing fat. In this study, applying the PC...
Influence of hygrothermal conditions on milk production in a free stall barn during hot weather
Presented are results of a study on the influence of air temperature and humidity on milk production by 174 Holstein-Friesian cows kept in a free stall barn. Occurrence of heat stress in three technological groups of cow...