Callus formation and establishment of cell suspensions sugarcane embryogenics from leaf segments in vitro plants
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 1
Abstract
The somatic embryogenesis, propagation technique and tool for the genetic improvement in sugar cane (Saccharum spp. hybrid), has presented multiple inconveniences that the regeneration protocols described in the literature have not been able to solve. The present research was carried out with the objective of developing a new methodology for the establishment of cells suspensions of sugar cane, var. C 87-51, somatic embryogenesis starting from segments of the sheath of the leaves of plants in vitro, placed directly in a liquid culture medium. The results demonstrated that it is possible to form callus with embryogenic structures starting from the segment two of the sheath of the leaves which reduces the time of culture in vitro and guarantees the physiologic and phytosanitary quality of the plants that are regenerated. Homogeneous lines of cellular suspensions formed by embryogenic agregates were established starting from callus with embryogenic structure.
Authors and Affiliations
Marisol Freire-Seijo, Rafael G. Kosky, Idalia Herrera Ofarril, Maritza Reyes
Keywords
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