In vitro establishment of buds of Ipomoea batatas
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 2
Abstract
Different buds of sweet potatowere studied. Roots of the clones Cemsa 78-354, Inivit 90-1, Inivit 93-1, Yabú-8 and Jewel were selected and put in glass with water in the laboratories for inducing the shoots. Afterwards the top shoot and the axiles shoots one to five (from top shoot ) were selected and sown in the Murashige and Skoog medium with the sales MS, Thiamine (1 mg.l-1), myoinositol (100 mg.l-1), sucrose (3%), gelrite (2 g.l-1) and different growth regulator combinations of giberelic acid (AG3) and 3-indol acetic acid (AIA), for example: AG3 (5 mg.l-1) + AIA (0.05 mg.l-1), AG3 (10 mg.l-1) + AIA (0.05 mg.l-1), AG3 (5 mg.l-1) + AIA (0.1 mg.l-1) and AG3 (10 mg.l-1) + AIA (0.1 mg.l-1). The pH was adjusted to 5.8. After five weeks, the morphologic behaviuor of the shoots was evaluated. The better results were obtained in the micropropagation with the use of the axiles shoots two, three and four in the culture medium whit growth regulator AG3 (10 mg.l-1) + AIA (0.05 mg.l-1).
Authors and Affiliations
Orlando S. González Paneque, Margarita Hernández Espinosa, Juan J. Silva Pupo, Silvia Montes Cruz, Mirtha López Machado, Antonio Sigarroa González
Keywords
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