Use of Temporary Immersion Systems for the large-scale production of in vitro tubers of Solanum tuberosum L. var. Atlantic and study of its behavior in the field
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 1
Abstract
A special temporary immersion system was designed in order to scale up the potato in vitro tubers production as an alternative method for potato seed production. An average of 2.6 in vitro tubers with an average fresh weight of 1.27g and diameter of 11.4mm was obtained. Only 2.9% of the in vitro tubers had a diameter of less than 4.0mm, while 78.1% of the in vitro tubers were larger than 7.0mm in diameter. These tubers can be stored and directly planted without an acclimatization stage so they are the most desirable for commercial production. Under field conditions, the plants obtained from in vitro tubers reached an average height of 22.77cm and 1.51 stems per plant compared to an average height of 15.32cm and 1.16 stems per plant reached by the in vitro plants. During the harvest the number of minitubers per plant was similar in both treatments, although comparing fresh weight and diameter, the results were statistical superior for in vitro tubers than for in vitro plants.
Authors and Affiliations
Naivy Pérez-Alonso, Manuel de Feria, Elio Jiménez, Alina Capote, Maité Chávez, Elisa Quiala
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