In vitro propagation in Temporary Immersion System of sugarcane plants variety ‘RB 872552’ derived from somatic embryos
Journal Title: Biotecnología Vegetal - Year 2015, Vol 15, Issue 3
Abstract
In this study, we used a temporary immersion system (TIS) to multiply sugarcane (Saccharum spp.) plants obtained by somatic embryogenesis (SE). SE was induced from immature leaf segments that were grown in culture medium supplemented with 2,4-D and BAP. Embryo formation occurred in 81% of the inoculated explants and 254 plants were regenerated. Ninety plants were transferred to TIS and cultured in medium supplemented with BAP. After three subcultures, 60 000 plantlets were obtained and transferred to rooting media. After 30 days of acclimatization period plantlets were well developed and exhibited a 96% survival. The results demonstrate the feasibility of the combined use of two important techniques of in vitro culture (SE and shoot multiplication in TIS) to sugarcane in vitro propagation.
Authors and Affiliations
Marina Medeiros de Araújo Silva, Emmanuel Cabral Medeiros, Gileno Vitor Mota Lima, Lilia Willadino, Terezinha Camara
Keywords
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