Can the antiplatelet effects of cangrelor be reliably studied in mice under in vivo and in vitro conditions using flow cytometry?
Journal Title: Pharmacological Reports - Year 2013, Vol 65, Issue 4
Abstract
The effects of blood platelet inhibitors are often not quite equivalent under in vivo and in vitro conditions. Amongst various models of human pathology using laboratory animals, mice offer several benefits that make them convenient tools for studying the putative therapeutic value of various compounds. However, despite its advantages, the mouse model has methodological limitations concerning the small amount of blood available and technical difficulties with its collection. Among the variety of available methods used to study blood platelet activation and/or reactivity, flow cytometry seems an attractive technique that largely minimizes the constraints of using small rodents and enables outcomes of laboratory research to be transferred successfully to clinical practice. In this study we aimed at a critical evaluation of the optimal discriminative flow cytometric protocol, useful for reliable studies of the effect of cangrelor, a P2Y12 receptor antagonist, on mouse platelets under in vitro and in vivo conditions.
Authors and Affiliations
Hassan Kassassir, Karolina Siewiera, Radosław Sychowski, Cezary Watała
Keywords
Related Articles
- EP ID EP99252
- DOI -
- Views 107
- Downloads 0
Cigarette smoke-induced biochemical perturbations in human erythrocytes and attenuation by epigallocatechin-3-gallate--tea catechin.
The protective effect of epigallocatechin-3-gallate (EGCG) against cigarette smoke (CS) induced alterations in human erythrocyte was studied using an in vitro model. Hemolysis, carboxyhemoglobin, osmotic fragility, hemin...
Modulation of chemical signaling: current problems after four decades of research. Symposium on the occasion of the retirement of Professor Manfred Göthert. Bonn [Germany], June 10, 2006.
Metabolic and monocyte-suppressing actions of fenofibrate in patients with mixed dyslipidemia and early glucose metabolism disturbances.
The aim of this study was to compare the action of fenofibrate on monocyte cytokine release between patients with isolated mixed dyslipidemia and dyslipidemia coexisting with prediabetic states in relationship with its m...
Transdermal buprenorphine in the treatment of cancer and non-cancer pain - the results of multicenter studies in Poland.
This was a multicenter, non-interventional, post-marketing study that aimed to evaluate the analgesic activity, safety of use, safety profile and adverse drug reactions of transdermal buprenorphine (Transtec® 35, 52.5 an...
Clinical aspects of assessment of endothelial function.
Prevention and treatment of atherosclerosis is an important priority. Measurement of endothelial function has been reported as a useful tool for atherosclerosis research. Endothelium appear to integrate the injury of com...