Causes of HLA-A2 reactive antibodies and analysis of their eplets
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 6
Abstract
Objective To analyze differences of eplets between the patient who generated HLA allele-specific antibodies after platelet transfusion with donors. Methods The HLA genotypes of the patient and donors were detected by PCR-SBT, and the Luminex single antigen beads coating was used to screen HLA-Ⅰ antibodies in the patient’s serum. HLA Matchmaker was utilized to analyze different amino acids and eplets. Results The patient carried HLA-A*02∶03 allele, and HLA-A2 antibodies were found in his serum after platelet transfusion (A*02∶01, A*02∶06, and A*02∶07). Sequence alignment showed that the patient′s A*02∶03 has a difference in position 149, which resulted in a different eplet between A*02∶03 and A*02∶01, A*02∶06, A*02∶07 and then induced the production of antibodies. Conclusion HLA antibodies are specific for HLA epitopes that have structural differences due to amino acid differences between HLA alleles, suggesting that high-resolution typing of HLA-A, -B need to be conducted in patients and donors, and the acceptable mismatch of HLA should be determined based on epitopes rather than antigens, so as to reduce alloimmune response and improve platelet count after transfusion.
Authors and Affiliations
Jingjing HUANG, Longhai TANG, Yuanling ZUO, Yan CAO, Weijie LI, Min JIANG, Mingyuan WANG
Keywords
Related Articles
- EP ID EP738559
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.06.002
- Views 68
- Downloads 0
Research progress of platelets and their derivatives based drug delivery systems
Platelets play an important role in physiological and pathological activities such as thrombosis, inflammation and tumorigenesis. At present, the application of platelets in drug delivery systems is increasingly studied....
HBV genotyping and population genetics analysis of voluntary blood donors in five regions of China
Objective To investigate the distribution of Hepatitis B virus(HBV)genotypes and the genetic characteristics of genotype B HBV populations among voluntary blood donors in five regions of China. Methods A total number of...
Adverse events in platelet-rich plasma collection and countermeasures
Objective To explore the quality of platelet-rich plasma(PRP) and adverse events during PRP collection and the countermeasures, so as to provide reference for the development of PRP therapy. Methods A total of 412 patien...
Four microcolumn agglutination anti-human globulin cards in unexpected antibody screening results: a comparative analysis
[Objective] To analyze the detection ability of one imported and three domestic microcolumn agglutination anti-human globulin cards in unexpected antibody screening test. [Methods] A total of 104 positive samples from an...
Exosomes and their dual role under viral infection
Exosomes are extracellular vesicles that are released from the cell into the extracellular microenvironment, and are a carrier of biological information transmitted over long distances, which have a dual regulatory effec...