CdTe Quantum Dots and Their Bioconjugate with Human Serum Albumin for Fluorescence Imaging
Journal Title: Фізика і хімія твердого тіла - Year 2017, Vol 18, Issue 2
Abstract
The interaction between CdTe quantum dots (QDs) with human serum albumin (HSA) and human cell culture was studied by optical spectroscopy technique. Performed research explored the interaction between the CdTe QDs and HSA, and fluorescence imaging efficiency of the QD-HSA bioconjugates in comparison with colloidal QDs. The secondary structure of the HSA is similar to the native form, which suggest a biocompatibility of prepared bionanocomplex. The CdTe QD-HSA bionanoconjugate shows chemical stability in phosphate-buffered saline (PBS) under ambient conditions, furthermore, it is stable in cytoplasm and suitable for cell labeling, tracking, and other bioimaging applications. The CdTe QDs located in an osteosarcoma cancer cells show a high luminescence intensity. The light emission of the CdTe QDs connected with albumin is less than the pure QDs, but it is still satisfactory, and additionally it is stable and have long photoluminescence lifetime. It suggest, that the CdTe NC-HSA bionanocomplex can be used as a fluorescent probe for cell labeling, tracking, and other bioimaging applications.
Authors and Affiliations
I. D. Stolyarchuk, R. Wojnarowska - Nowak, J. Polit, E. Sheregii, S. Nowak, M. Romerowicz - Misielak
Keywords
Related Articles
- EP ID EP264067
- DOI 10.15330/pcss.18.2.166-172
- Views 86
- Downloads 0
Problems of Selection of Lubricants for Ethylene High-Pressure Compressors. 4. Investigation of Antifriction Properties of Naphtene and Polyglycol Oils during High Loading of Solid Lubricant Pair
The antifriction properties of naphtene and polyglycol oils during little loading with lubrication of pair bronze-tungsten-carbide-alloy WK-6 (WK-ll) and graphelon-20-WK-6 (WK-11) and high loading with lubrication of pai...
Magneto-Optical Properties of CdMnTe Diluted Magnetic Semiconductor Nanoparticles Prepared by Physical Methods
Nanoparticles of Cd1-xMnxTe have been studied by optical absorption and photoluminescence in applied magnetic field. The pulsed laser deposition technique and ball milling were used for fabrication of the samples of dilu...
Optical Properties of Ferrite Powders of NixCo1-xFe2O4 System
The aim of this work was to create and study of ferrite nickel-cobalt powders, using sol-gel technology with participation of auto-combustion. It is studied the optical properties of the powders depending on the degree o...
Habit, Symmetry and Orientation of Surface Structures Vapor-Phase Condensation SnTe: Bion Ceramics
The analysis was obtained by atomic force microscopy method of topograms nanostructures on the surface of vapor-phase condensation of SnTe: Bi, thet were opened deposited by evaporation in a vacuum on substrates of ceram...
Magneto- and Tensoresistance of the p-Ge Compensated Crystals in the Range of Weak, Intermediate and Classically Strong Magnetic Fields
On the crystalsof compensatedp-Ge (with the compensation factor of k = NSb/NGa = 0,5) the transverse (Н (J // X)) magnetoresistance (within the magnetic fields of 0 < Н 22.3 kE) at fixed values of the mechanical stresses...