Cell death and survival following manual and femtosecond laser-assisted capsulotomy in age-related cataract
Journal Title: International Journal of Ophthalmology - Year 2018, Vol 11, Issue 9
Abstract
"AIM: To study molecular and morphological changes in lens epithelial cells following femtosecond laser-assisted and manually performed continuous curvilinear capsulotomy (CCC) in order to get information about these methods regarding their potential role in the induction of development of secondary cataract. METHODS: Anterior lens capsules (ALC) were removed from 40 patients with age-related cataract by manual CCC and by femtosecond laser-assisted capsulotomy (FLAC). Samples removed by manual CCC were assorted in group 1, FLAC samples were classified in group 2. Morphology of lens epithelial cells was examined with light and electron microscopes. Following capsulotomy, expressions of p53, Bcl-2 and cyclin D1 genes were analyzed with reverse transcriptase polymerase chain reaction. Immunohistochemistry was used to detect the pro-apoptotic p53 in the epithelial cells. RESULTS: Light and electron microscopic examination showed that ALC of group 1 contained more degenerating cells following manual CCC than after FLAC. The expression level of p53 was higher after manual than laser-assisted surgery. Immunocytochemistry indicated significantly higher number of cells containing p53 protein in the manual CCC group than following FLAC. Bcl-2 and cyclin D1 gene expression levels were slightly lower following manual CCC than after FLAC, but the difference was not significant. CONCLUSION: Manually removed ALC shows slightly, but not significantly larger damage due to the mechanical stretching and pulling of the capsule than those removed using FLAC."
Authors and Affiliations
Zsolt Biró
Keywords
Related Articles
- EP ID EP479549
- DOI 10.18240/ijo.2018.09.02
- Views 45
- Downloads 0
Artificial intelligence on diabetic retinopathy diagnosis: an automatic classification method based on grey level co-occurrence matrix and naive Bayesian model
"AIM: To develop an automatic tool on screening diabetic retinopathy (DR) from diabetic patients. METHODS: We extracted textures from eye fundus images of each diabetes subject using grey level co-occurrence matrix metho...
Understanding practice patterns of glaucoma sub-specialists in India
"AIM: To obtain information on the prevailing practice patterns of glaucoma specialists in India. METHODS: Glaucoma specialists attending the Annual Conference of the Glaucoma Society of India (GSI) were surveyed. This s...
Optical coherence tomography angiography of optic disc perfusion in non-arteritic anterior ischemic optic neuropathy
"AIM: To compare the optic disc blood flow of non-arteritic ischemic optic neuropathy (NAION) eyes with normal eyes. METHODS: The optic disc blood flow densities of diagnosed non-acute phase NAION eyes (21 eyes, 14 indiv...
Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide
"AIM: To explore the effect of ciliary neurotrophic factor (CNTF) on retinal ganglion cell (RGC)-5 induced by hydrogen peroxide (H2O2). METHODS: After cell adherence, RGC-5 culture medium was changed to contain different...
Preliminary study on visual recognition under low visibility conditions caused by artificial dynamic smog
"AIM: To quantitatively evaluate the effect of a simulated smog environment on human visual function by psychophysical methods. METHODS: The smog environment was simulated in a 40×40×60 cm3 glass chamber filled with a PM...