Cellulase immobilized on copolymers based on N-vinylformamide: Influence of immobilization on the catalytic activity of the enzyme
Journal Title: CHEMIK nauka-technika-rynek - Year 2015, Vol 69, Issue 8
Abstract
Immobilization effect of cellulase from Aspergillus sp. EC 3.2.1.4. catalytic activity in reaction of enzymatic hydrolysis of cellulose has been studied. Cellulase immobilized on a hydrophilic polymer carriers with formamide groups and primary amino groups. The carriers were synthesized in a free radical copolymerization of N-vinylformamide (NVF) with divinylbenzene (DVB). The reactive primary amino groups in the copolymer were generated by decarboxylation formamide groups in an alkaline medium. Cellulase was covalently immobilized via glutaraldehyde (GA) on the support with amino groups and adsorbed on a support with formamide groups. The resulting heterogeneous biocatalysts tested in the hydrolysis reaction microcrystalline cellulose. It was found that immobilized cellulase activity on both carriers tested was greater than the enzyme in the native state.
Authors and Affiliations
K Sokołowska, A Konieczna-Molenda, E Witek
Keywords
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