CHANGES IN LEVELS OF ANTIOXIDANT MINERALS AND VITAMINS IN WISTAR MALE RATS EXPOSED TO METHIONINE CONTAINING ACETAMINOPHEN FORMULATION
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 5
Abstract
This study was carried out to determine the effect of toxic and subtoxic doses of acetaminophen on antioxidant vitamins and minerals in male Wistar rats. Five groups served as the test groups and received different doses of the acetaminophenmethionine formulation in ratio 9:1. The sixth group served as the control and received only the vehicle: physiologic saline. At the end of 24 hours of exposure, blood was obtained from each rat through retro-orbital bleeding, the levels of antioxidant vitamins and minerals were determined using high-performance liquid chromatography and atomic absorption spectrophotometric technique. Results showed that at 100 mg/kg BW level of exposure, niacin, vitamin A, vitamin E, zinc, copper, manganese, selenium were significantly increased (p< 0.05) whereas riboflavin was not significantly different (p>0.05) compared with controls. At both 350 and 1000 mgkg BW levels of exposure, all these antioxidant indices were significantly decreased (p<0.05) except niacin which was not significantly altered (p>0.05) at 350 mgkg BW (body weight) level of exposure compared with the control group. The animals in 3000 mgkg BW and 5000 mgkg groups suffered 40% and 100% mortality respectively by the end of the 24th hour. This study therefore showed that acetaminophenmethionine formulation in the ratio of 9:1 may cause alteration of vital elements and biomolecules not only at toxic levels but also at tolerable level of 100 mgkg BW.
Authors and Affiliations
Ayobola Iyanda , John Anetor , Francis Adeniyi
Keywords
Related Articles
- EP ID EP114075
- DOI -
- Views 85
- Downloads 0
SIMULTANEOUS ESTIMATION AND VALIDATION OF VARDENAFIL AND DAPOXETINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION BY THIN LAYER CHROMATOGRAPHIC DENSITOMETRIC METHOD
The present manuscript describes new, simple, accurate, and precise high performance thin layer chromatography method for the simultaneous determination of Vardenafil and Dapoxetine in combined tablet dosage form. Chroma...
FORMULATION AND EVALUATION OF EXTENDED RELEASE TABLETS OF TRAMADOL HYDROCHLORIDE
The objective of this research work was to develop extended release tablets (Twice in a day) of Tramadol Hydrochloride using different Hydrophilic polymers like HPMC K15M, HPMC K4M, Metalose 60SH50, Carbopol 971P, Sodium...
RECENT POTENTIAL USAGE OF SURFACTANT FROM MICROBIAL ORIGIN IN PHARMACEUTICAL AND BIOMEDICAL ARENA: A PERSPECTIVE
The use and potential commercial application of biosurfactant has increased during the past decade which can be used as emulsifiers, de-emulsifiers, wetting and foaming agents, functional food ingredients and as detergen...
PRELIMINARY PHYTOCHEMICAL EVALUATION OF WHOLE PLANT EXTRACT OF DIPTERACANTHUS PROSTRATUS NEES
Dipteracanthus prostratus is an prostrate perennial herb. It is a small straggling, much branched herb it is purple at the nodes, internodes are long and hairy. It used as anti-cancer, hypoglycemic, anti-inflammatory and...
SALT STRESS TOLERANCE AND STRESS PROTEINS IN WHEAT (Tritium Aestivum L.)
One of the unique properties of living organisms is growth. Growth is a complex phenomenon and represents the end result of metabolic pathways bringing about an overall irreversible change. Growth is the final morphologi...