Changes in the Activity of Antioxidant and Glyoxylate Cycle Enzymes of Hydro-Primed Calendula officinalis (L.) Seeds after Re-Drying Temperature Stress
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2013, Vol 9, Issue 2
Abstract
In order to study the effect of re-drying temperatures on the enzymatic activation of hydro-primed [i]Calendula officinalis[/i] (L.) seeds, a completely randomized design with three replications was conducted. Treatments were six different re-drying temperatures including control (without drying), 20, 30, 40, 50, and 60 °C applied after hydro-priming. Results showed that rapid drying at high temperatures (40-60 °C) caused a significant difference comparing control. The best re-drying temperature considering many studied traits was 20-30 °C. At 60 °C, activity of antioxidant enzymes such as superoxide dismutase, catalase and peroxidase decreased 65.5, 75, and 62%, respectively over control. Re-drying had not significant effect on the activity of malate synthase and isocitrate lyase in comparison with control and 20-30 °C that demonstrates no negative and reductive effect of re-drying with these temperatures on seed germination. It seems that seeds are more desiccation tolerant at these temperatures. In conclusion, we can dry hydro-primed seeds slowly at 20 and 30 °C and store them until utilization.
Authors and Affiliations
Mohammad Sedghi
Keywords
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