Changes of the histomorphological structure of the bursa of fabricius in chiken after immunization with immunocomplex live vaccines against Gumboro disease
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 31, Issue
Abstract
Introduction. Infectious bursal disease (Gumboro disease, IBD) is a viral infection, characterized by diarrhea, intramuscular hemorrhages, destrution of the bursa of Fabricius, digestive tract and kidneys. The disease is extremely contagious, affects young chickens up to 130- days age. In infected flocks, morbidity is high, with up to 100%. The death rate is about 40-60%. It is well-known fact that vaccination is the only correct and reliable solution in the disease control. The goals of the work was to determine changes in the histomorphic structure of the bursa after vaccination with immunocomplex live vaccines. Materials and methods. For the research, two groups of 1-day old SPF chickens (specific pathogen free) were formed. The first group was vaccinated subcutaneously in one 0.2 cm3 dose. The control group was not vaccinated. After 28 days, each chick was infected by intra-ocular administration of the IBD virulent strain. Within 5 days after a virus provocation, the animals were slaughtered by decapitation after a chloroform anesthesia for internal organs sampling for pathomorphological studies. The organs samples were fixed in 10% neutral formalin solution, embedded in paraffin and histological preparations were made. Histological examination of each samples was conducted and calculated the level of lesion of the bursa using the scale according to the European Pharmacopoeia. ВЕТЕРИНАРНА БІОТЕХНОЛОГІЯ 31, 2017 32 Results of research and discussion. The microscopic structure of bursa in the control and experimental group has a vacuolar dystrophy, microplasticity and cysts of epithelium of the mucous membrane, partial or complete exhaustion of the follicles, replacement of parenchymal and stromal structures with dense fibrous connective tissue, and their infiltration with lymphocytes and pseudoeosinophiles. The changes in the bursa of the control group chickens were more pronounced. Сonclusions and recommendations for further research. The results of the research suggest the histological method as the main ones to determine the efficacy of the studied live and attenuated vaccines against Gamboro disease.
Authors and Affiliations
Natalia Gavrasieva, A Kuzmich, Artem Kostiyk
Keywords
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- EP ID EP265993
- DOI 10.31073/vet_biotech31-03
- Views 165
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