CHARACTERISATION OF PORPHYROMONAS-PREVOTELLA GROUP ISOLATED FROM ORODENTAL INFECTIONS AND ANTIMICROBIAL ACTION OF HERBAL EXTRACTS
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 57
Abstract
BACKGROUND Porphyromonas–Prevotella group includes non-motile, non-sporing, black pigmented Gram-negative coccobacilli. Even though they are commensals of oral cavity, many species are associated with a variety of orodental as well as invasive human infections. As these isolates are exhibiting resistance to many antibiotics, an attempt is done in this study to evaluate antimicrobial activity of various herbal extracts from natural sources. Objectives- To characterise Porphyromonas-Prevotella group isolated from orodental infections by biochemical reactions and to screen the antimicrobial action of various herbal extracts against these isolates. MATERIALS & METHODS Clinical samples such as subgingival plaques, paper points, saliva were directly inoculated in Reduced Transport Fluid and Robertson’s Cooked Meat Medium, subcultured onto culture media using a semiquantitation technique under anaerobic conditions employing BD GasPak system. After incubation, colonies morphologically resembling Porphyromonas–Prevotella group were speciated by biochemical reactions. Antimicrobial activity was performed against various herbal extracts on supplemented Brucella Blood Agar by Kirby-Bauer disc diffusion method. Seven herbal extracts - Betel leaf, Clove, Ginger, Lemon grass, Mango leaves, Nutmeg, Peppermint were employed in this study. RESULTS Speciation of 43 isolates of Porphyromonas-Prevotella group revealed Porphyromonas gingivalis, Porphyromonas asaccharolytica, Prevotella intermedia, Prevotella melaninogenica and Prevotella buccae. All 43 isolates showed variable activity to the herbal extracts. Among the seven extracts, clove oil, lemongrass oil and nutmeg oil showed good activity. Betel leaf extract was inactive against these isolates while Ginger, Mango leaves and Peppermint showed variable activity. CONCLUSION Porphyromonas-Prevotella group includes important pathogens encountered in orodental infections. The results of this preliminary study is a promising finding for developing an alternate treatment regimen, without side effects, for the control of orodental infections caused by Porphyromonas and Prevotella group.
Authors and Affiliations
Beena Antony, Chinu Maria Cyriac, Jinsha K, Ramanath Karicheri
Keywords
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- EP ID EP226164
- DOI 10.14260/Jemds/2017/918
- Views 101
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