Characteristics of coagulation process in cows during the pregnancy and postpartum periods and in cows with postnatal and obstetrical pathology
Journal Title: Біологія тварин - Year 2016, Vol 18, Issue 4
Abstract
The proper functioning and adaptation of the hemostatic system is important for the physiological course of pregnancy. Monitoring system for the assessment of blood clotting is not yet fully developed in veterinary medicine. That is why the main goal of the research was to adapt the coagulation methods of laboratory diagnostics and determine their information usefulness while assessing the state of the hemostasis system in pregnant cows at the 35th day, the 3, 5, 7, 8th month of pregnancy and 2–3 days before birth, after birth and during the course of сhronic postpartum metritis and hypotrophy of the ovaries. The screening tests — prothrombin time and activated partial thromboplastin time (APTT) which are designed to characterize the external and internal pathways of blood coagulation, as well as methods for the determination of fibrinogen, soluble fibrin monomer complex (SFMC), products of fibrinogen degradation (FDP), degradation products of fibrinogen (PRF), the activity of protein C have been validated. The use of these techniques has allowed determining the imbalance between pro- and anticoagulant functioning of blood clotting system in cows. The analysis of the state of the blood clotting system of pregnant cows showed that prothrombin index, fibrinogen level, PRF and protein C activity at all stages of pregnancy and calving period was within the normal limits. The detected reduced clotting time of plasma in the APTT test at the third month of pregnancy and during the postpartum period were accompanied by the appearance in plasma small amount of SFMC (0.001 to 0.01 mkg/ml) which are a specific markers for the activation of blood coagulation system. Coagulation processes in cows with сhronic postpartum metritis and hypotrophy of the ovaries were characterized by an increased number of markers of thrombophilia — SFMC (from 0.005 to 0.12 mkg/ml) and PRF, a significant reduction in activity of the protein C in 70 % of animals, and a tendency to a decreased fibrinolytic potential (up to 0.41 u/ml at a rate of 1–1.3 ml u/ml). It was found that the informative tests for detecting the activation of the blood clotting system in cows are the determination of the content of soluble fibrin monomer complexes, functionally inactive form of prothrombin and protein C activity. The algorithm for diagnosis of thrombophilia in cows may also include the tests for determining the concentration of fibrinogen, PRF and fibrinolytic potential.
Authors and Affiliations
S. Vlasenko, M. Rublenko, T. Chernyshenko, O. Gornitska
Keywords
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- EP ID EP261695
- DOI 10.15407/animbiol18.04.014
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