CHARACTERISTICS OF MICROBIOLOGICAL PARAMETERS OF THE FROZEN FISH IN THE PRESENCE OF ANTIBIOTIC RESIDUES
Journal Title: ВЕТЕРИНАРІЯ, ТЕХНОЛОГІЇ ТВАРИННИЦТВА ТА ПРИРОДОКОРИСТУВАННЯ - Year 2018, Vol 2, Issue
Abstract
Due to its high nutritional and biological value, fish is a good nutrient for development of microorganisms, therefore, the conditions and terms of its storage require appropriate temperature regimes to stop the growth of microflora. The work aimed at determining the microbiological parameters of frozen fish, which according to biochemical parameters was designated as stale, depending on the presence of antibiotic residues. In samples, we studied mesophilic microorganisms at temperature (30 ± 1) ºС incubation of crops during 72 hours and psychrotrophic microflora at temperature (6.5 ± 0.5) ºС incubation - during 10 days. Bacteria of the colibacillus group were determined in Endo and Kesler's medium, and staphylococcus aureus in saline haemo-agar. Biochemical parameters: reaction with copper sulfate, peroxidase, pH was determined using generally accepted methods, total volatile content of nitrogen is in accordance with EU Regulation 2027/2005. Presence of antibacterial residues was determined using microbiological and immuno-enzymatic methods. It was established that samples of frozen fish which according to the biochemical parameters designated it as stale, as well as absence of antibacterial residues in the flesh, were mainly contaminated by psychotrophic microflora in the amount of over 1 mil CFU/g. According to the content of colibacillus group the number of fish samples which fit the standard (up to 1 thousand CFU/g or absence in 0,001 g of the product) was 4.8 ± 0.2%, and according to the content of staphylococcus aureus it was 9.7 ± 0,3% (standard up to 100 CFU/g or absence in 0,01 g of the product). Number of tests according to the content of coliforms, which exceeded 10 thousand CFU/g, was 88.7 ± 3.4%, which is 5.8 times (p <0,05) more than number of samples with such a content of staphylococcus aureus. Stale frozen fish, according to biochemical parameters, produced staphylococcus aureus in the amount from 1 thousand to 10 thousand CFU/g in 62,1 ± 2,7% of cases. It was established that number of fish samples designated as poor quality ones according to their biochemical parameters, but which according to the content of antibiotics, according to the content of mesophilic microflora met standard requirements were in average 14 times (p <0.05) more compared with the fish samples without antibiotic residues. It was revealed that according to the contents of bacteria of colicacilus group and Staphylococcus aureus this fish in 90,9 ± 2,7% of samples met the requirements of ISO, which correspondently is 18.9 and 9.3 times (p <0.05) more compared to such fish without antibiotics. It was proved that quantitatively psychrotrophic microflora of the frozen fish exceeds the amount of MAPANM and more fully characterizes biochemical processes which determine its freshness. Thus, only comprehensive control of frozen fish imported into Ukraine, including biochemical, microbiological parameters and presence of antibacterial residues will allow to detect and eliminate dangerous products.
Authors and Affiliations
Z. V. Malimon
Keywords
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- EP ID EP455718
- DOI 10.31890/vttp.2018.02.25
- Views 122
- Downloads 0
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