CHARACTERIZATION OF CARBAPENEM RESISTANT ACINETOBACTER BAUMANNII ISOLATED IN A TERTIARY CARE HOSPITAL: EPIDEMIOLOGY AND TREATMENT OUTCOME
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: Carbapenem resistant Acinetobacter baumannii (CR-Ab) has emerged as a major nosocomial pathogen, but optimal treatment regimens are unknown. Our objectives were to determine the epidemiology and outcome of CR-Ab infections at a tertiary care hospital.Methods: CR-Ab isolates were collected from January to April 2013. MICs were determined and isolates were subjected to screening for carbapenemase production by Modified Hodge test (MHT), metallo-β-lactamase (MBLs) by EDTA disk synergy test and AmpC β-lactamase by AmpC disk test. 15 isolates were subjected to PCR for detection of resistant genes, blaOXA-23, blaVIM and blaNDM. Treatment outcomes of infections were evaluated.Results: 51 CR-Ab isolates from tracheal aspirate (21), blood (15); tissue/wound/drainage (13) and urine samples (2) were collected. Colistin appeared to be the most effective agent with 98% in vitro activity. MHT showed 98% positivity, MBLs production was detected in 94.1% isolates and 64.7% were positive for AmpC β-lactamase production. All 15 isolates carried blaOXA-23 and blaVIM, of these 3 also carried blaNDM gene. Colistin containing combinations were more commonly used (68.3%). Colistin-noncarbapenem combination showed improved clinical response compared to colistin-carbapenem combination against Acinetobacter isolates carrying blaOXA-23 and blaVIM.Conclusion: A stringent infection control practice along with antimicrobial stewardship is needed to prevent emergence of Acinetobacter carrying multiple carbapenemase genes along with blaNDM. Various colistin combinations are preferentially used to treat CR-Ab infections. Identification of antimicrobial combinations with proven in vitro activity that encompass local susceptibility patterns as well as molecular mechanisms of resistance is needed to provide better outcome.Keywords: Acinetobacter baumannii, Carbapenem resistance, Carbapenemases, Colistin combination, metallo-β-lactamase, NDMÂ
Authors and Affiliations
Mehta Pooja B. , Shah Sweta R. , Kamat Sheela D. , Kamat Dileep V.
Keywords
Related Articles
- EP ID EP576255
- DOI -
- Views 97
- Downloads 0
INHIBITORY EFFECTS OF ANCHUSA AZUREA EXTRACTS ON XANTHINE OXIDASE ACTIVITY AND ITS HYPOURICEMIC EFFECTS ON MICE
Objective: The aim of the present study was to determine the effects of the polyphenols and flavonoids from Anchusa azurea on XO activities in vitro and on serum and liver uric acid levels in normal and potassium oxonate...
SCREENING AND CHARACTERIZATION OF DEVELOPING RESISTANT CULTIVAR AGAINST ODOIPORUS LONGICOLLIS (OLIVIER) (COLEOPTERA: CURCULIONIDAE) USING REFERENCE GENOTYPES IN INDIA
Objective: The objective of the research was to develop a screening method for weevil resistance by using selected genotypes. The selected reference genotypes were screened against stem weevil to deals with the different...
DEVELOPMENT AND VALIDATION OF A RAPID AND SIMPLE REVERSED-PHASE HPLC METHOD FOR THE DETERMINATION OF GEMCITABINE IN HUMAN PLASMA
Objective: In order to investigate the human plasma pharmacokinetics of dFdC, the objective of this work was to optimize and validate a rapid reversed-phase (RP) high-performance liquid chromatography (HPLC) method accor...
SYNTHESIS OF TRIAZOLOTHIADIAZINE DERIVATIVES AS ANTIOXIDANT AGENTS
Objectives: The objective of the present study is to synthesize 3-substituted phenyl-6-phenyl-7H-[1, 2, 4] triazolo [3, 4-b][1,3,4] thiadiazine. The structures of all the synthesized compounds were characterized by IR, 1...
ANTIOXIDANT ACTIVITY AND CYTOTOXIC POTENTIAL OFHYDROALCOHOLIC EXTRACT OF SALACIA FRUTICOSA (ROOT)-IN VITRO
Objective: To evaluate the antioxidant and cytotoxic potential of hydroalcoholic extract of Salacia fruticosa Heyne ex Lawson-root by various In-Vitro methods.Methods: The various concentration of hydroalcoholic extract...