CHARACTERIZATION OF HIGH ACTIVITY DRUG-STIMULATED ATPase OF MULTIDRUG RESISTANT LEISHMANIA DONOVANI AMASTIGOTE CELLS
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2015, Vol 4, Issue 6
Abstract
Background: The emergence of drug resistance and the limited knowledge of the mechanisms by which the parasites acquire resistance are the major obstracle for the control of leishmaniasis. Methods: The present investigation is aimed to study multidrug resistance related ATPase activity in in vitro generated sodium stibogluconate (SSG) and paramomycin (PMM) resistant Leishmania donovani amastigote permeablized by digitonin Results: The results of the multidrug resistance related ATPase activity in SSG and PMM resistant L.donovani amastigotes revealed that ATPase activities were strongly stimulated by resistance causing drugs, and these ATPase stimulation activities had broad specificities. Conclusion: Generation of drug resistance in L. donovani amastigotes leads to concurrent development of drug-stimulated ATPase activity.
Authors and Affiliations
Chowdhury S| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India, Roy A| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India, Kar N| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India, Chakraborty S| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India, Bera T| Division of Medicinal Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India
Keywords
Related Articles
- EP ID EP16032
- DOI -
- Views 395
- Downloads 17
Assessment of Home Based Newborn Care provided by ASHA worker in a Rural Block of Haryana
Objective: The first month of life, the post-natal period, for the mother and the newborn is the most critical time in the life. Based on these facts Government of India took an initiative, Home Based Post Natal Care (...
Speciation, biofilm formation and antifungal susceptibility of Candida isolates
Recently, an increase in the incidence of infections caused by fungi especially non-albicans Candida species has been reported. Several virulence factors like biofilm formation, toxin production and presence of adhesin...
Preparation and evaluation of Maltodextrin based Proniosomes containing Capecitabine
Maltodextrin based capecitabine proniosomes were fabricated by conventional slurry method with different surfactant: cholesterol molar ratio keeping constant capecitabine and dicetyl phosphate concentration. The fabric...
REGULATION FOR DEVELOPMENT OF BIOSIMILAR : A REVIEW
Present article signifies the exigency for regulation and regulatory bodies involved in development of biosimilars. The principle for development of biosimilars included opting adequate reference product, manufacturing...
Antioxidant properties of polyphenolic rich HPLC standardized extract of Beta vulgaris L. roots
OBJECTIVES: The present study was planned to evaluate the antioxidant effects of standardized Beta vulgaris L. or beetroot. METHODS: The polyphenols present in lyophilized water extract of beetroot (BR) were quantifie...