Characterization of Immunophenotyping in ALL: A Single Center Study of Western India
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2016, Vol 4, Issue 9
Abstract
This retrospective observational study was done among 227 ALL cases at a cancer treatment center in Western India. Data were collected retrospectively from hospital records during June 2015 to May 2016. Diagnosis of ALL was made based on the com¬plete blood cell counts, peripheral blood smear/bone marrow aspirate smear and immunophenotyping study. Out of 227 ALL patients, 217 cases were of B-ALL, 8 cases were of T-ALL and 2 cases were of biphenotypic ALL. Of these 217 cases of B-ALL 190 (87.56) were pediatric age (range 0.5-15 years) and 27 (12.44) were adult (range 16-52 years). T-ALL cases were equally distributed in both age groups, 4 (50%) cases in pediatric age group (range 1-7 years)and 4 (50%) cases were adult (range 19-62 years). biphenotypic leukemia found in only children (2 (100%) case, one was 3 year old male and another was 5 years age male). The frequency of B-cell marker in B-ALL was found to be 217 (100%) for CD 19, 193 (88.94%) for CD 10, 162 (74.65%) for CD 79a and 35 (16.13%) for CD 20. CD 34 expression was found in 37 (17.05%) cases of B-ALL. HLA-DR was found in 30 (13.82%) cases while TdT was found in 27 (12.44%) cases. Aberrant expression of myeloid antigens was found in 57 (26.27%) cases of B-ALL. Among T-ALL, the positivity of CD 3 and CD 7 was 100 % (8/8 cases) while CD 5 was positive in 6 (75%) cases. CD 34 expression was found in only one case of T-ALL. Two cases of ALL had biphenotypic leukemia with positive CD3, CD 7, CD 19, CD 20, HLA-DR and TdT. There was aberrant expression of myeloid antigen in B-ALL. CD 13 was found in 37 (17.05%) B-ALL and CD 33 was found in 20 (9.22%) B-ALL cases. None of the T-ALL case had aberrantly expressed myeloid marker. We concluded that immunophenotyping plays a key role in diagnosis of ALL. Keywords: ALL, Immunophenotyping study, B-ALL, T-ALL, Biphenotyping leukemia
Authors and Affiliations
Dr. Sandeeep K Jasuja, Dr Sandhya Gulati, Shraddha Patel, Dr Nidhi Sharma
Keywords
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