Characterization of Mutations in the Rpob and Katg Gene of Mycobacterium Tuberculosis Isolates From Pasteur Institute of Tehran
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 8
Abstract
Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the bsubunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of katg gene. The rpoB and katG sequences of 30 isolates were analyzed to identify the mutations and compare the mutations with their related susceptibilities. Results: In this research, we investigated the location and type of rpoB and katG mutations in Mycobacterium tuberculosis which had been achieved from Pasteur Institute of Tehran. PCR Amplification and DNA sequencing methods were performed. In this assay, from 507 to 537 codons and 315 codons of rpoB and katG genes were sequenced and also mutations were analyzed, respectively.
Authors and Affiliations
Hamid Tebyanian| Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran, Hamze farhadian| Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran, Mohamad Reza Imen Shahidi| Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran, Mehdi Behdani| Biotechnology Research Center, venom & Biotherapeutics Molecules Lab., Pasteur Institute of Iran, Tehran, Iran
Keywords
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