Characterization of non albicans candida species and the antifungal susceptibility profile of clinical isolates
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 3
Abstract
Introduction and Objectives: Antibiotics, immunodeficiencies and advances in medical practices have all contributed to increased incidence in fungal infections. In recent times Non albicans Candida species are increasingly being isolated from clinical specimens. The present study was aimed at the characterization and antifungal susceptibility of Non albicans Candida species from clinical specimens received in Microbiology Laboratory for one year from June 2015–May 2016. Materials and Methods: A total of 181 Non albicans Candida isolates were included in the study. Candida was identified from clinical samples by gram stain, culture on Saboraud’s Dextrose Agar (SDA) and germ tube test. The organisms were further speciated by cornmeal agar morphology, sugar assimilation and fermentation tests and growth on CHROM agar (Himedia). Antifungal sensitivity was performed by using disc diffusion method as per CLSI guidelines. Results: The most common isolate was Candida tropicalis (59.11%) followed by C.glabrata (28.18%), C.parapsilosis (8.83%) and C.krusei (3.88%). Highest number of isolates were from urine (74/181). Resistance to Fluconazole in clinical isolates of C.tropicalis has increased. C.krusei was the most resistant species. Conclusion: Use of CHROM agar is rapid, simple and cost effective compared to time consuming technically demanding expensive conventional methods. Antifungal susceptibility testing has to be carried out before starting therapy because of varying susceptibility pattern in different species of Candida.
Authors and Affiliations
Lekshmi Balaraman, Saritha N, Geetha Raveendran
Keywords
Related Articles
- EP ID EP471545
- DOI 10.18231/2394-5478.2018.0062
- Views 81
- Downloads 0
Bacteriological profile of post surgical wound infections: A one year retrospective study from a government orthopaedic hospital
Post-operative wound infection is a severe problem in the surgical specialization and is an important cause of morbidity and mortality. In most of these wound infections the causative agents arise from the endogenous flo...
Role of semi-quantitative bacteriological culture in management of chronic wound infections
Introduction: The role of quantitative microbiology in the management of wound infections is very crucial. In this study semi-quantitative swab technique is used as a simple procedure for assessing the bacterial load of...
Antibiotic Sensitivity Pattern of Salmonella Typhi in a Stand Alone Lab in Central Madhya Pradesh
Introduction: Typhoid fever or enteric fever, is an endemic disease in many developing countries specially India and the Indian Subcontinent. In the past decade, resistance of Salmonella to multiple drugs is on the rise...
A study of aerobic bacteriological profile of chronic suppurative otitis media in a tertiary care hospital, South India
Introduction: In the Otolaryngology Out Patient Department (OPD), Chronic Suppurative Otitis Media (CSOM) is the most common encountered otological disease. Staphylococcus aureus and Pseudomonas species are the most comm...
Biofilm production by staphylococcus aureus and staphylococcus epidermidis: an evaluation of three different screening methods
Purpose: The purpose of this study was to determine the rate of biofilm production by S.aureus and S.epidermidis isolated from different clinical specimens. Methods: Quantitation of biofilm formation by staphylococci was...