Characterization of Polish feline B. henselae isolates by multiple-locus tandem repeat analysis and pulse-field gel electrophoresis
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2012, Vol 19, Issue 1
Abstract
Knowledge about molecular epidemiology of [i]B. henselae[/i] is important for recognizing the geographical distribution of strains and identification of isolates virulent for humans. Eleven Polish feline [i]B. henselae[/i] isolates were typed, using 2 different techniques: pulse-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA). PFGE analysis distinguished 6 different PFGE types, with subtypes within 3 of them, whereas 10 MLVA types were assigned. Global diversity index (D.I.) for MLVA equaled 0.93. For 7 isolates, the results of MLVA confirmed cluster assignments based on PFGE. Both PFGE and MLVA results were in accordance with epidemiological data. Although PFGE has been previously demonstrated to be a suitable method for the differentiation of [i]B. henselae[/i] isolates/strains, our results show the superiority of MLVA over PFGE with respect to higher discriminatory power, distinguishing genotypes I and II isolates, easier analysis of results, and possibility to compare the numerical data obtained by different laboratories. With MLVA, 7 new profiles were observed, compared to previous results from around the world; whereas 3 known profiles were previously described mainly in European [i]B. henselae[/i] isolates. Our results confirm that some VNTR profiles can be used as specific geographical markers.
Authors and Affiliations
Edyta Podsiadly, Nadia Haddad, Moez Berrich, Rim Bouchouicha, Benott Durand, Martine Monteil, Henri-Jean Boulouis, Stanislawa Tylewska-Wierzbanowska
Keywords
Related Articles
- EP ID EP80427
- DOI -
- Views 147
- Downloads 0
<strong><em>Borrelia burgdorferi</em></strong><strong> sensu lato as activators of the complement system in <em>in vitro</em> model.</strong>
Introduction. The following parameters were analyzed: C5a, which is significant in classical and alternative pathways of the complement system activation, and factor H, the major function of which is to regulate the alte...
Seasonal variations of allergenic pollen in a Mediterranean region – Alexandroupolis, north-east Greece
[b]Objectives.[/b] The aim of this study was to describe the aerobiological characteristics of NE Greece, identify the most prevalent taxa, define the duration of the main pollen season and correlate allergen records wit...
Managing water safety in healthcare. Part 2 – Practical measures and considerations taken for waterborne pathogen control
These summaries form the second part of presentations made at a recent conference held at the Royal Society for Public Health in London, 16-17 May 2012, on the latest developments in combating waterborne hospital-acquire...
<strong>Acute respiratory distress syndrome (ARDS) complicating influenza <em>A/H1N1v</em> infection - a clinical approach.</strong>
ARDS is defined as an acute inflammatory syndrome characterized with bilateral parenchymal lung infiltrates on chest radiograph and PaO[sub]2[/sub]/FiO[sub]2[/sub] ratio&amp;lt;200 resulting from causes other than ac...
Release of bisphenol A and its derivatives from orthodontic adhesive systems available on the European market as a potential health risk factor
[b]Introduction[/b]. Treatment with fixed orthodontic appliances requires the application of adhesive systems to enable secure fastening of brackets and retainers to the surface of tooth enamel. The orthodontic bonding s...