Checking of the clostridium chauvoei R-15 production strain typicality according to morphophiziological parameters
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 30, Issue
Abstract
Introduction. It has been made the analysis of international normative documents base concerning preparation of microorganisms’ strains for industrial applying (using as pattern Clostridium chauvoei), storage of them without change of passport descriptions, and control of major properties of species. The major standardization stages of the main quality indicators of aluminium hydroxide formol-vaccine against blackleg of cattle and sheep using the strain Cl. chauvoei R-15 are shown, in particular, morphological, tinctorial, cultural, biochemical, immunological and virulent features. The purpose of the work was to: a) evaluate the properties of strain Cl. chauvoei R-15, pointed in state documentation base, in compare with international requirements in relation to qualityof vaccine; b) define directions of improvement of vaccine preparation. Materials and methods of researches. As a research material the production strain Cl. chauvoei R-15 was used. In the researches we used bacteriological, biological, biochemical methods. We conducted a study of the viability, activity and stability of the passport characteristics of the refreshed strain stored in a deeply frozen state for 11 years (preservative 30% glycerol solution) and lyophilized culture Cl. chauvoei R-15. Pathogenicity of productive strain of Cl. chauvoei R-15 studied on guinea-pigs (mass 400±50 g) in the dose of 0.5 ml and 4 months calf. Results of research and discussion. The studies showed that the production strain Cl. chauvoei R-15 (native and cultural forms) showed its typical morphological features, tinctorial, cultural, biochemical, toxigenic (as a biomodel white mice were used) and virulent (as a biomodel guinea pigs were used) properties. After infecting the calf, pathological material (heart, spleen, liver, kidneys, muscles) was collected during the first hour after death for bacteriology to study Cl. chauvoei R-15 typicality by morphological signs and biocamical properties. Conclusions and prospects of further researches. Productive strain Cl. chauvoei R-15 after either passage through the live organism or storage lyophilized had saved typical and stable morphology feathers, tinctorial, physiology and pathology properties and can make use without additional passage through the organism of susceptible animals for production of concentrated hydroxide aluminum formolvaccine against blackleg as the effective veterinary immunobiological preparation.
Authors and Affiliations
Larisa Аkimenko, Serghiy Nychyk, Peter Boyko, O. Boyko, Alexander Assori
Keywords
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- EP ID EP267256
- DOI 10.31073/vet_biotech30-02
- Views 105
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