Effectiveness of oral immunization of dogs against rabies using vaccine “Brovarabis V-RG”
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 30, Issue
Abstract
Introduction. In most developing countries, the dog is the principal vector of rabies transmission to humans, despite the widespread use of parenteral vaccination. After demonstrations of the oral vaccination effectiveness in wild carnivores in many countries, including Ukraine, have showed the possibility of creating a persistent rabies immunity and intermission of the epizootic cycle of rabies among foxes (particularly in Europe), current studies are aimed at developing a similar approach for the control of rabies among dogs. The goal of the work. To study the effectiveness of rabies vaccine “Brovarabies V-RG” for oral immunization of carnivores which is used for dogs.Materials and methods. 10 mongrel dogs of 1-2 years old, weighing 6-20 kg were used for vaccination with vaccine “Brovarabies V-RG”. Animals were divided into groups: four dogs were fed one dose of the vaccine “Brovarabies V-RG”; two dogs were fed 2 baits in 7 days interval, twodogs were fed 5 baits (one bait at the beginning of experiment, 2 baits after 7 days and the rest 2 baits after the next 7 days). On the 14th, 30th and 90th days after rabies vaccination (first feeding bait vaccine) blood samples were taken and serum were obtained. Blood serum was tested for antibodies against rabies using the FAVN-test (Fluorescent antibody virus neutralization). The research results were expressed in International units (IU/ml). Results of research and discussion. On the14th day after a single feeding of baits with vaccine the titer of antibodies against rabies was 1.87 IU/ml. On the 90th day of the experiment, the titer of rabies antibodies decreased slightly and was 1.15 IU/ml, which is typical in the formation of rabies immunity.In the second and third groups of animals, which were fed two doses of vaccine, antibody titers were higher. So, on the 14th day the levels of rabies antibody ranged from 3.95 to 5.51 IU/ml. However, higher titers of specific antibodies were detected on the 30th and the 90th day from dogs that received two doses of vaccine with 7 days interval and were 7.63 IU/ml and 6.48 IU/ml respectively. The highest titers of rabies antibodies were found in the fourth group of animals that were fed five doses of vaccine within 14 days on the 30th and 90th day 93.10 IU/ml and 53.58 IU/ml respectively. Conclusions and prospects for further research. Rabies vaccine “Brovarabies V-RG” for oral immunization of carnivores consumed good by dogs and formed rabies immunity during research period (90 days). Taking into account the results it is necessary to continue researching of the dynamics of rabies antibodies within a year and conducting field tests of vaccine “Brovarabies V-RG” for dogs.
Authors and Affiliations
Nikolai Golik, Vitalii Nedosekov, J. Sharai, Serghiy Nychyk, Ivan Polupan
Keywords
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- EP ID EP267274
- DOI 10.31073/vet_biotech30-04
- Views 96
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