CHIKUNGUNYA VIRUS INFECTION EVALUATION USING RAPID DIAGNOSTIC TESTS (RDTS) BASED ON ANTI-CHIKUNGUNYA IGM/IGG
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2019, Vol 6, Issue 4
Abstract
Chikungunya virus (CHIKV) is a single-stranded RNA alphavirus. Recently, CHIKV began an unprecedented global expansion. Thus, it is necessary to develop commercial rapid diagnostic kits for CHIKV infection. We assessed the utility of the commercially available blood tests approved by the rapid diagnostic tests (RDTs) of GenBody Company (Korea) for the detection of CHIKV. In addition, to detect the presence of viral genomic RNA in human samples, RT-PCR of CHIKV was done. RT-PCR of CHIKV was shown the typical banding profile of the rapid diagnostic test for 30 cases of CHIKV negative sera with primers targeting a 557-bp region of the E2 gene. 29 sera were distinct bands (96.6% sensitivity). In this study, the 470 samples were submitted to the Focus Diagnostics Reference Laboratory for CHIKV RNA and/or CHIKV antibody (IgG and IgM). Sensitivity of the GenBody Company RDT was 87.5% (105/120) for IgG and its specificity was 98.8% (316/320). 40 samples were IgM-antibody positive. Sensitivity of the GenBody Company RDT was 74.1% (40/54) for IgM and its specificity was 97.5% (312/320). Most IgM-positive sera were also IgG positive (145/174 = 83.3%). 16.7% were positive for IgM-antibody but negative for ELISA/RT-PCR. It needs to make a new analysis kit such as RNA composition for the detection of anti-chikungunya IgM.
Authors and Affiliations
Yong Lim
Keywords
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