Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 3
Abstract
Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chloride channel blocker (DIDS) and curcumin on Mcf-7 and human normal cell viability. Patch-clamp technique was used to determine the current density before and after drug treatment. Apoptosis assay by flow cytometry was performed for further examination of cell apoptosis. Results: Curcumin had toxicity on Mcf-7 and HUVEC cells and DIDS reduced the survival rate of Mcf-7 cells by inhibiting proliferation. Curcumin could activate the chloride ion current on MCF-7 cell membrane, which would be inhibited by DIDS. Finally, curcumin in low concentration combined with DIDS could significantly promote the MCF-7 cells apoptosis. Conclusions: Our results suggest that ClC-3 protein is involved in the regulation of curcumin induced proliferation inhibiting in breast cancer cells through inducing cell apoptosis. ClC-3 may be a potential target of tumor therapy.
Authors and Affiliations
Zheng Wu, Xi Lin
Keywords
Related Articles
- EP ID EP275197
- DOI 10.4103/1995-7645.228440
- Views 65
- Downloads 0
In silico identification and characterization of common epitope-based peptide vaccine for Nipah and Hendra viruses
Objective: To explore a common B- and T-cell epitope-based vaccine that can elicit an immune response against encephalitis causing genus Henipaviruses, Hendra virus (HeV) and Nipah virus (NiV). Methods: Membrane proteins...
Cytotoxic, antioxidant and antimicrobial activities of Nerium oleander collected in Morocco
Objective: To investigate the cytotoxic, antioxidant and antibacterial activities of ethanolic and aqueous crude extracts of Nerium oleander (N. oleander) leaves. Methods: Cytotoxic activities were evaluated by WST-1 bio...
Chemoprotective activity of aqueous leaf extract of Acalypha wilkesiana against cyclophosphamide-induced toxicity in rats
Objective: To investigate the protective effect of aqueous leaf extract of Acalypha (A.) wilkesiana Muell. Arg (Euphorbiaceae) against cyclophosphamide-induced toxicity in albino rats. Methods: Twenty male albino rats we...
Chemical analysis and antioxidant content of various propolis samples collected from different regions and their impact on antimicrobial activities
Objective: To assess the antioxidant content, antimicrobial and antioxidant activities of various propolis samples. Methods: Seven propolis samples were collected from different locations in Morocco, which are characteri...
Wild chive oil is an extremely effective larvicide against malaria mosquito vector Anopheles stephensi
Objective: To assess the chemical composition and mosquito larvicidal potentials of essential oil of wild chive (Allium schoenoprasum L.) against Anopheles stephensi. Methods: In the search for an environmentally safer...