CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF FLAVONOIDS OF ACHYRANTHES ASPERA LINN. BY HPTLC

Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 4

Abstract

The study was carried out with an aim to determine the chemical profile and flavanoid composition of the medicinally important plant Achyranthes aspera Linn. from methanolic extract of root, stem and leaf. Preliminary phytochemical screening was done followed by HPTLC studies. Ethyl acetate: Formic acid: Glacial acetic acid : water 10:0.5:0.5:1.3 was used as mobile phase for the separation of flavanoids. The flavanoid fingerprint of leaf exhibited 9 peaks each in methanolic neutral, acidic and basic fractions, while that of root exhibited 8 in neutral, 11 in acidic and 7 in basic methanolic extracts. The fingerprint of stem revealed the presence of 8 peaks in neutral, 9 in acidic and 7 in basic methanolic fractions. The study revealed diverse forms of flavonoids in large number in the root, stem and leaf of Achyranthes aspera Linn. It can be concluded that HPTLC fingerprint analysis of root, stem and leaf extract of Achyranthes aspera Linn. can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker.

Authors and Affiliations

Aparna Saraf*| Department of Botany, The Institute of Science, 15, Madame Cama Road, Fort, Mumbai, India, and Aruna Samant| Department of Botany, The Institute of Science, 15, Madame Cama Road, Fort, Mumbai, India

Keywords

Related Articles

THE POSSIBLE PROPHYLACTIC EFFECT OF EGYPTIAN MORINGA OLEIFERA SEED OIL AS ANTI-ULCER ACTIVITY AND ANTI-INFLAMMATORY IN RATS.

Moringa oleifera Lam. is the multipurpose tree (therapeutic potential and nutritive value). In this work, authors focused on importance of Moringa oleifera seed oil (MSO) as antioxidant, anti-inflammatory and antiulcer....

Assessment, Monitoring and Reporting Adverse Drug Reactions of Corticosteroids: A Prospective Observational Study

Background: Corticosteroids are widely used medication for the treatment of various conditions. Using too little dose of steroids can lead to sub therapeutic response whereas using higher dose of steroids can lead to Adv...

CLINICO-ETIOLOGICAL STUDY OF NEONATAL JAUNDICE IN A TERTIARY CARECENTRE IN AMBALA (HARYANA) INDIA

Jaundice occurs in most newborn infants. Most jaundice is benign, but because of the potential toxicity of bilirubin, newborn infants must be monitored to identify those who might develop severe hyperbilirubinemia and, i...

A REVIEW ON SOLID SELF EMULSIFYING DRUG DELIVERY SYSTEM

Self-emulsifying drug delivery system (SEDDS) is one of the most popular and commercially viable formulation approaches for enhancing solubility of poorly water soluble drugs. SEDDS are isotropic mixtures of oil, surfac...

Recent Advances in Novel Semisolid Dosage Forms: An Overview

Semisolids constitute a significant proportion of pharmaceutical dosage forms. They serve as carriers for drugs that are topically delivered by way of the skin, cornea, rectal tissue, nasal mucosa, vagina, buccal tissue,...

Download PDF file
  • EP ID EP3013
  • DOI -
  • Views 442
  • Downloads 20

How To Cite

Aparna Saraf*, and Aruna Samant (2014). CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF FLAVONOIDS OF ACHYRANTHES ASPERA LINN. BY HPTLC. Journal of Biomedical and Pharmaceutical Research, 3(4), 13-24. https://europub.co.uk/articles/-A-3013