Chromosomal localization of genes encoding small heat shock proteins (HSPB) in cattle and sheep
Journal Title: Journal of Animal Science, Biology and Bioeconomy - Year 2015, Vol 33, Issue 4
Abstract
The small heat shock protein gene products (HSPB) reveal chaperone and neuroprotective activity contributing to the stabilization of cell homeostasis and cytoskeleton of neurons in conditions of thermal or oxidative stress. The aim of this study was chromosomal localization of five small heat shock protein genes (HspB1, HspB2, CRYAB – alternative name HspB5, HspB6 and HspB8) in cattle and sheep, with an application of FISH technique with the probes obtained from BAC clones (derived from the CHORI-240 Bovine BAC Library) containing sequences of these genes. Prior to in situ hybridization, carried out on metaphase chromosomes stained by means of DAPI bands technique, the presence of the studied genes in the selected clones was confirmed by PCR method with the use of the gene-specific primers. As a result of the experiments, FISH signals in the following cattle (BTA) and sheep (OAR) genome regions were obtained: BTA25q22/OAR24q22 (HSPB1), BTA15q14-21/OAR15q14-21 (HSPB2 and CRYAB), BTA18q24/OAR14q24 (HSPB6), BTA17q24-25/OAR17q24-25 (HSPB8). The studies enabled to designate physical localization of the studied genes on the genome maps of these species and confirmed the high level of autosome conservation in Bovidae. The results obtained may provide useful information concerning the genetic background of neurodegenerative diseases in breeding animals.
Authors and Affiliations
Barbara Danielak-Czech, Anna Kozubska-Sobocińska, Marek Babicz
Keywords
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