Circulating Serum Levels of Fox P3, GATA-3 and IL-17 A as Potential Biomarkers in Patients with Symptomatic Asthma
Journal Title: Journal of Communicable Diseases - Year 2019, Vol 51, Issue 2
Abstract
Background: The lack of information on the association of certain factors in the pathophysiology of the chronic inflammatory disorder, Asthma limits its early detection and therapy. Keeping these facts in view, an endeavour was made to investigate the roles, if any, such as IL-17A, FoxP3 and GATA-3 in the occurrence and intensity of asthma in north Indian population. Objective: The study was conducted to evaluate the level of FoxP3, GATA-3 and IL-17A in symptomatic asthma patients as compared to healthy controls. Methods: In the study we had taken 125 cases and 125 controls. Levels of total interleukin 17A, transcription factors FoxP3 and GATA-3 were measured by ELISA in serum of asthmatic patients. Student’s T-test and ANOVA test was applied for quantitative variables. The qualitative variables analyzed using Chi-Square test /Fisher’s exact test.Results: Clinical symptoms like breathlessness, cough, headache, disturbed sleep, congestion, and wheezing were found significantly elevated in asthmatics as compared to the controls. Except haemoglobin; in the cases the levels of blood eosinophils, Absolute Eosinophil Count (AEC), Total Leukocyte Count (TLC) and serum Immunoglobulin E (IgE) were observed higher as compared to the controls and also the higher serum levels of IL-17A and GATA-3 were found in the cases. Contrary to IL-17A and GATA-3; the levels of FoxP3 was increased in the control, and was lower in the patient serum. A positive correlation was found between total IgE and GATA-3 (Pearson Correlation=0.283, P=0.042). We have shown that GATA-3 is increased in the asthmatic patients (0.541±0.140) than in the controls (0.312±0.076). The GATA-3 was found over expressed in patients with severe asthma.Conclusion: Increased level of GATA-3 and IL-17A might be useful as diagnostic marker for patients with symptomatic asthma there is need for further larger scale studies to establish the role of these biomarkers in asthmatic patients
Authors and Affiliations
Rashmi Pandey, Ved Prakash, Sheetal Verma, SN Sankhwar, Md. Kaleem Ahmad
Keywords
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- EP ID EP667748
- DOI 10.24321/0019.5138.201914
- Views 99
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