CLINICO-MYCOLOGICAL STUDY OF DERMATOPHYTIC SKIN INFECTIONS IN A TERTIARY CARE CENTRE
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 8
Abstract
BACKGROUND Skin infections are the most common infections occurring in developing countries, of which dermatophytosis are of particular concern in tropics. The prevalence of dermatophytic infections varies with different geographic areas and climatic conditions. Hence, identification of aetiological agents is important for diagnosis and treatment as well as for studying epidemiological characteristics in a region. Objectives1. To isolate and identify the dermatophytes infecting skin. 2. To compare two different culture media, namely Sabouraud’s Dextrose Agar (SDA), Hi-Media Laboratories, Mumbai) with Actidione and Dermatophyte Test Medium (DTM, Hi-Media Laboratories). MATERIALS AND METHODS This is a cross-sectional study conducted in patients attending the Outpatient Department of Dermatology and Venereology, Government Medical College, Kottayam, Kerala, India, with clinical features of dermatophytic skin infections from January 2009 - December 2009. Skin scrapings were subjected to direct microscopy by 10% potassium hydroxide (KOH) and cultured on SDA with Actidione (Hi-Media Laboratories) and DTM (Hi-Media Laboratories). RESULTS The total number of samples in this study was 115, of which 76 (66.1%) samples were positive by direct microscopy and 50 (43.5%) were positive by culture. The most common clinical type was tinea corporis 46 (40%). Out of the 50 isolates, Trichophyton rubrum 24 (48%) was the most common species followed by Trichophyton mentagrophytes 20 (40%), Epidermophyton floccosum 4 (8%), Trichophyton violaceum 1 (2%) and Microsporum gypseum 1 (2%). Nearly, 85.4% of the dermatophytes were isolated on DTM within 5 - 10 days of incubation, whereas 45.7% were isolated on SDA with Actidione within 5 - 10 days of incubation. CONCLUSION DTM can be used as a rapid screening medium for the isolation and identification of dermatophytes compared to SDA with Actidione. But DTM is inferior to SDA with Actidione for species level identification of dermatophytes.
Authors and Affiliations
Shanimole Puthenpurayil Ebrahimkutty, Shoba Kurian Pulikkottil, Dani Kochuplapparambil Thampi
Keywords
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- EP ID EP410100
- DOI 10.14260/jemds/2018/230
- Views 56
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